Hybridoma cell strain, monoclonal antibody for resisting PDCoV NS6 protein and application of monoclonal antibody

The invention discloses a hybridoma cell strain and an anti-PDCoVNS6 protein monoclonal antibody secreted by the hybridoma cell strain, and the anti-PDCoVNS6 protein monoclonal antibody is an NS6 protein which has high specificity and can specifically recognize PDCoV, and can be used for detecting porcine delta coronavirus. The invention also discloses an application of the hybridoma cell strain in preparation of a reagent, a kit or a test strip of the monoclonal antibody containing the anti-PDCoVNS6 protein. The invention also discloses a reagent, a kit or a test strip containing the hybridoma cell strain and/or the monoclonal antibody. The invention also discloses application of the hybridoma cell strain and/or the monoclonal antibody and/or the reagent, the kit or the test strip in immunodetection of the porcine delta coronavirus. 本发明公开了一株杂交瘤细胞株和由所述杂交瘤细胞株分泌产生的抗PDCoVNS6蛋白的单克隆抗体，所述抗PDCoVNS6蛋白的单克隆抗体一种具有高特异性、能够特异识别PDCoV的NS6蛋白，可用来检测猪δ冠状病毒。本发明还公开了所述杂交瘤细胞株在制备含抗PDCoVNS6蛋白的单克隆抗体的试剂、试剂盒或试纸条中的应用。本发明还公开了一种试剂、试剂盒或试纸条，含