Preparation method of D-psicose
The invention relates to the technical field of biology, in particular to a preparation method of D-psicose, which comprises the following steps: (1) taking D-fructose as a substrate, adding a solvent and a first whole-cell catalyst, adjusting the pH value of a reaction system to be weakly acidic, c...
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| creator | WANG LEI TAO YONG LIAO FEI WEI PINGPING HU MEIRONG ZHU LIPING CHEN KECAI CHENG SHUANGWU |
| description | The invention relates to the technical field of biology, in particular to a preparation method of D-psicose, which comprises the following steps: (1) taking D-fructose as a substrate, adding a solvent and a first whole-cell catalyst, adjusting the pH value of a reaction system to be weakly acidic, controlling the temperature of the reaction system, and reacting for 2-4 hours to obtain D-psicose; after the reaction is finished, centrifugally collecting supernate, namely a D-allicin alcohol solution; and (2) taking the D-alliinol solution in the step (1), adjusting the pH value to be neutral, adding a second whole-cell catalyst in a ventilating and stirring state, and reacting to obtain the psicose solution. By adopting the method, the conversion rate of the D-fructose to the D-psicose is greatly improved.
本发明涉及生物技术领域，具体涉及一种D-阿洛酮糖的制备方法，该制备方法包括以下步骤：(1)以D-果糖为底物，加入溶剂和第一全细胞催化剂，调整反应体系的pH为弱酸性，控制反应体系的温度，待反应结束后离心收集的上清液即为D-蒜糖醇溶液；(2)取步骤(1)中的D-蒜糖醇溶液，调pH至中性，在通风、搅拌状态下再加入第二全细胞催化剂，反应结束后得到阿洛酮糖溶液。采用上述方法大大提高了D-果糖向D-阿洛酮糖的转化率。 |
| format | Patent |
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本发明涉及生物技术领域，具体涉及一种D-阿洛酮糖的制备方法，该制备方法包括以下步骤：(1)以D-果糖为底物，加入溶剂和第一全细胞催化剂，调整反应体系的pH为弱酸性，控制反应体系的温度，待反应结束后离心收集的上清液即为D-蒜糖醇溶液；(2)取步骤(1)中的D-蒜糖醇溶液，调pH至中性，在通风、搅拌状态下再加入第二全细胞催化剂，反应结束后得到阿洛酮糖溶液。采用上述方法大大提高了D-果糖向D-阿洛酮糖的转化率。</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZJAPKEotSCxKLMnMz1PITS3JyE9RyE9TcNEtKM5Mzi9O5WFgTUvMKU7lhdLcDIpuriHOHrqpBfnxqcUFicmpeakl8c5-hoZmhmZGZmaWjsbEqAEAFDwktA</recordid><startdate>20230526</startdate><enddate>20230526</enddate><creator>WANG LEI</creator><creator>TAO YONG</creator><creator>LIAO FEI</creator><creator>WEI PINGPING</creator><creator>HU MEIRONG</creator><creator>ZHU LIPING</creator><creator>CHEN KECAI</creator><creator>CHENG SHUANGWU</creator><scope>EVB</scope></search><sort><creationdate>20230526</creationdate><title>Preparation method of D-psicose</title><author>WANG LEI ; TAO YONG ; LIAO FEI ; WEI PINGPING ; HU MEIRONG ; ZHU LIPING ; CHEN KECAI ; CHENG SHUANGWU</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN116162669A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>WANG LEI</creatorcontrib><creatorcontrib>TAO YONG</creatorcontrib><creatorcontrib>LIAO FEI</creatorcontrib><creatorcontrib>WEI PINGPING</creatorcontrib><creatorcontrib>HU MEIRONG</creatorcontrib><creatorcontrib>ZHU LIPING</creatorcontrib><creatorcontrib>CHEN KECAI</creatorcontrib><creatorcontrib>CHENG SHUANGWU</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>WANG LEI</au><au>TAO YONG</au><au>LIAO FEI</au><au>WEI PINGPING</au><au>HU MEIRONG</au><au>ZHU LIPING</au><au>CHEN KECAI</au><au>CHENG SHUANGWU</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Preparation method of D-psicose</title><date>2023-05-26</date><risdate>2023</risdate><abstract>The invention relates to the technical field of biology, in particular to a preparation method of D-psicose, which comprises the following steps: (1) taking D-fructose as a substrate, adding a solvent and a first whole-cell catalyst, adjusting the pH value of a reaction system to be weakly acidic, controlling the temperature of the reaction system, and reacting for 2-4 hours to obtain D-psicose; after the reaction is finished, centrifugally collecting supernate, namely a D-allicin alcohol solution; and (2) taking the D-alliinol solution in the step (1), adjusting the pH value to be neutral, adding a second whole-cell catalyst in a ventilating and stirring state, and reacting to obtain the psicose solution. By adopting the method, the conversion rate of the D-fructose to the D-psicose is greatly improved.
本发明涉及生物技术领域，具体涉及一种D-阿洛酮糖的制备方法，该制备方法包括以下步骤：(1)以D-果糖为底物，加入溶剂和第一全细胞催化剂，调整反应体系的pH为弱酸性，控制反应体系的温度，待反应结束后离心收集的上清液即为D-蒜糖醇溶液；(2)取步骤(1)中的D-蒜糖醇溶液，调pH至中性，在通风、搅拌状态下再加入第二全细胞催化剂，反应结束后得到阿洛酮糖溶液。采用上述方法大大提高了D-果糖向D-阿洛酮糖的转化率。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Preparation method of D-psicose |
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