METHOD FOR EDITING TARGET DNA, METHOD FOR PREPARING CELL EDITED WITH TARGET DNA, AND DNA EDITING SYSTEM USED THEREFOR

A method for editing a target DNA, which comprises a step of bringing (1) a fusion protein that contains TALE and a nucleobase converting enzyme, and (2) a CRISPR-Cas9 system that contains a Cas9 protein that partially or entirely loses nuclease activity and a guide RNA thereof into contact with a target DNA, and editing a base at a target site of the target DNA by the nucleobase converting enzyme activity of the fusion protein, a TALE recognition sequence recognized by TALE in the fusion protein or a complementary sequence of the TALE recognition sequence exists on the 5'side of the target site through a spacer region 1 with the chain length of 7-31 bp, and a guide RNA target sequence recognized by guide RNA in the CRISPR-Cas9 system exists in a mode of containing a complementary basic group of the target site. 一种编辑目标DNA的方法，其包括：使(1)含有TALE和核碱基转化酶的融合蛋白、以及(2)含有部分或全部丧失核酸酶活性的Cas9蛋白及其向导RNA的CRISPR-Cas9系统与目标DNA接触，通过所述融合蛋白的核碱基转化酶活性，编辑所述目标DNA的目标位点的碱基的工序，所述融合蛋白中的TALE所识别的TALE识别序列或其互补序列经由7～31bp的链长的间隔区1存在于所述目标位点的5′侧，并且所述C