Method and kit for detecting swine influenza virus based on CRISPR-Cas and application

The invention discloses a method and a kit for detecting a swine influenza virus based on CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats) and application. According to the detection method, swine influenza virus specific crRNA is designed on the basis of the H1HA gene of the swine influenza virus, meanwhile, a CRISPR Cas fluorescence detection system is combined, whether the swine influenza virus exists or not can be detected in a short time, currently known swine influenza virus disease species can be covered, a detected object is covered with a larger area, and the method has the advantages of being simple, good in specificity, high in sensitivity, high in sensitivity and the like. And the lowest detection limit can reach 10 < 8 >-10 < 9 > copies/[mu] L. 本发明公开了一种基于CRISPR-Cas检测猪流感病毒的方法、试剂盒及应用；本发明检测方法基于猪流感病毒的H1HA基因，设计了猪流感病毒特异性crRNA，同时结合CRISPR Cas荧光检测系统，可以短时间检测出是否存在猪流感病毒，且可以覆盖目前已知的猪流感病毒病种，更大面积的覆盖了检测对象，具有方法简单、特异性好、灵敏度高，最低检测限可达108～109copies/μL。