Primer combination, kit and method for detecting genetic typing of human neutrophil antigen

The invention relates to the technical field of molecular biological detection, in particular to a primer combination, a kit and a method for detecting human neutrophil antigen genotyping. A forward primer, a reverse primer and an extension primer are designed aiming at all 11 SNP loci of a human gr...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	LI LILAN, CHEN JIERUN, LU FANG, JIANG LIHONG, LI HENGCONG, ZHOU YAN, MO HUIHUI, WU GUOGUANG, ZHONG ZHOULIN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention relates to the technical field of molecular biological detection, in particular to a primer combination, a kit and a method for detecting human neutrophil antigen genotyping. A forward primer, a reverse primer and an extension primer are designed aiming at all 11 SNP loci of a human granulocyte antigen HNA 1-5 system, the sequences are shown as SEQ ID No: 1-22, a method for detecting human neutrophile granulocyte antigen genotyping by applying the primer combination can realize batch detection of 96 or 384 samples in each batch, and the detection efficiency is greatly improved. High-throughput detection brings great convenience to related clinical diagnosis and scientific research work, the experiment time is greatly saved, and the detection method is accurate and stable. 本发明涉及分子生物学检测技术领域，具体涉及用于检测人类中性粒细胞抗原基因分型的引物组合、试剂盒及方法。本发明针对人类粒细胞抗原HNA 1-5系统全部11个SNP位点设计了正向引物、反向引物和延伸引物，序列如SEQ ID No:1-22所示，应用所述引物组合检测人类中性粒细胞抗原基因分型的方法，可实现每批96个或384个样品的批量检测，高通量检测将为相关临床诊断和科研工作带来很大的便利，大大节约实验时间，且检测方法准确、稳定。