Method for detecting new coronavirus and variant strain by using double-stranded DNA (deoxyribonucleic acid) and colorimetric biosensor and application of method
The invention belongs to the technical field of molecular diagnosis, particularly relates to double-stranded DNA and a colorimetric biosensor for detecting new coronavirus and variant strains, and constructs a colorimetric biosensor for detecting SARS-CoV-2 with strong specificity and low cost on th...
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| creator | SHI KAI ZHANG YING YI ZHIGANG LEI XINMENG SONG JIUHUA ZHANG LIN |
| description | The invention belongs to the technical field of molecular diagnosis, particularly relates to double-stranded DNA and a colorimetric biosensor for detecting new coronavirus and variant strains, and constructs a colorimetric biosensor for detecting SARS-CoV-2 with strong specificity and low cost on the basis of Toehold mediated strand displacement reaction. Under the driving of thermodynamic entropy, target nucleic acid is combined with a Toehold region on double-stranded DNA to initiate strand displacement reaction, a complete G-rich sequence is released and is combined with hemin to form a G-quadruplex/hemin compound, and then hydrogen peroxide is catalyzed to oxidize 3, 3 ', 5, 5'-tetramethyl benzidine, so that the solution is blue. Meanwhile, a camera is adopted to capture RGB of the solution, and experimental results observed by naked eyes are verified. According to the invention, qualitative analysis can be carried out on SARS-CoV-2 target nucleic acid, and the sensing system shows high-specificity signif |
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Under the driving of thermodynamic entropy, target nucleic acid is combined with a Toehold region on double-stranded DNA to initiate strand displacement reaction, a complete G-rich sequence is released and is combined with hemin to form a G-quadruplex/hemin compound, and then hydrogen peroxide is catalyzed to oxidize 3, 3 ', 5, 5'-tetramethyl benzidine, so that the solution is blue. Meanwhile, a camera is adopted to capture RGB of the solution, and experimental results observed by naked eyes are verified. 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Under the driving of thermodynamic entropy, target nucleic acid is combined with a Toehold region on double-stranded DNA to initiate strand displacement reaction, a complete G-rich sequence is released and is combined with hemin to form a G-quadruplex/hemin compound, and then hydrogen peroxide is catalyzed to oxidize 3, 3 ', 5, 5'-tetramethyl benzidine, so that the solution is blue. Meanwhile, a camera is adopted to capture RGB of the solution, and experimental results observed by naked eyes are verified. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE |
| title | Method for detecting new coronavirus and variant strain by using double-stranded DNA (deoxyribonucleic acid) and colorimetric biosensor and application of method |
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