Primer group, kit and method for detecting carrier GATV3 transformation event
The invention relates to a safety evaluation and detection method of transgenic plants in the technical field of bioengineering, in particular to a primer group, a kit and a method for detecting a carrier GATV3 transformation event. The primer group comprises a left boundary specific primer and/or a...
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| creator | WU YONGZHONG WANG JIANHUA ANPO HIKARU HUANG PEIJING ZENG XIANG JIN XIONGXIA LI XINPENG |
| description | The invention relates to a safety evaluation and detection method of transgenic plants in the technical field of bioengineering, in particular to a primer group, a kit and a method for detecting a carrier GATV3 transformation event. The primer group comprises a left boundary specific primer and/or a right boundary specific primer; wherein the left boundary specific primer comprises GATV3-LB-R1/R2/R3, and the sequences of the GATV3-LB-R1/R2/R3 are respectively shown as SEQ ID NO: 1-3 in sequence; the right boundary specific primer comprises GATV3-RB-F1/F2/F3, and the sequences of the GATV3-RB-F1/F2/F3 are respectively shown as SEQ ID NO: 4-6 in sequence. The left and right flanking sequences of the GATV3 can be accurately and rapidly found out, specific bands cannot be expanded in non-transgenic plants or other transgenic plants, and the GATV3 marker is suitable for detecting, monitoring and safely managing the maintainer line GATV3 of the rice genetic intellectualized breeding technology (GAT) and derivative |
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The primer group comprises a left boundary specific primer and/or a right boundary specific primer; wherein the left boundary specific primer comprises GATV3-LB-R1/R2/R3, and the sequences of the GATV3-LB-R1/R2/R3 are respectively shown as SEQ ID NO: 1-3 in sequence; the right boundary specific primer comprises GATV3-RB-F1/F2/F3, and the sequences of the GATV3-RB-F1/F2/F3 are respectively shown as SEQ ID NO: 4-6 in sequence. The left and right flanking sequences of the GATV3 can be accurately and rapidly found out, specific bands cannot be expanded in non-transgenic plants or other transgenic plants, and the GATV3 marker is suitable for detecting, monitoring and safely managing the maintainer line GATV3 of the rice genetic intellectualized breeding technology (GAT) and derivative</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Primer group, kit and method for detecting carrier GATV3 transformation event |
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