Newcastle disease virus attenuated strain for expressing infectious bursal disease virus variant VP2 protein as well as preparation method and application of Newcastle disease virus attenuated strain
The invention discloses a Newcastle disease virus attenuated strain for expressing infectious bursal disease virus variant VP2 protein as well as a preparation method and application of the Newcastle disease virus attenuated strain. The attenuated strain is constructed by inserting an infectious bur...
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| creator | FANG JINGZHEN YU ZEKUN LI YANLI WANG TINGTING ZHOU SHENG LI SIFEI DUAN XIAOXIAO LUAN ZHIFANG |
| description | The invention discloses a Newcastle disease virus attenuated strain for expressing infectious bursal disease virus variant VP2 protein as well as a preparation method and application of the Newcastle disease virus attenuated strain. The attenuated strain is constructed by inserting an infectious bursal disease virus variant VP2 protein gene between P-M genes of an NDV gene VII type attenuated strain rSL strain. The preparation method comprises the following steps: firstly introducing a restriction enzyme cutting site between P-M protein genes of a genome main plasmid of an NDV gene VII type attenuated strain rSL strain, and then inserting a sequence containing an infectious bursal disease virus variant VP2 protein gene and the restriction enzyme cutting site between the P-M protein genes in a homologous recombination manner to construct a complete recombinant genome main plasmid; and co-transfecting BHK cells by using the main plasmid and three auxiliary plasmids for respectively expressing N, P and L protein |
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The attenuated strain is constructed by inserting an infectious bursal disease virus variant VP2 protein gene between P-M genes of an NDV gene VII type attenuated strain rSL strain. The preparation method comprises the following steps: firstly introducing a restriction enzyme cutting site between P-M protein genes of a genome main plasmid of an NDV gene VII type attenuated strain rSL strain, and then inserting a sequence containing an infectious bursal disease virus variant VP2 protein gene and the restriction enzyme cutting site between the P-M protein genes in a homologous recombination manner to construct a complete recombinant genome main plasmid; and co-transfecting BHK cells by using the main plasmid and three auxiliary plasmids for respectively expressing N, P and L protein</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE |
| title | Newcastle disease virus attenuated strain for expressing infectious bursal disease virus variant VP2 protein as well as preparation method and application of Newcastle disease virus attenuated strain |
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