Gene for auxiliary identification of southern soybean leaf wrinkling disease, molecular marker primer group and detection method
The invention discloses a gene for auxiliary identification of southern soybean leaf wrinkling disease, the base sequence of the gene is shown as SEQ ID NO.1, the gene can be stably expressed in a leaf wrinkling environment and is slightly influenced by other adverse conditions, and a molecular mark...
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| creator | LIANG JIANG GUO XIAOHONG TANG FUYUE CHEN WENJIE NING DEJIAO WEI QINGYUAN CHEN YUAN |
| description | The invention discloses a gene for auxiliary identification of southern soybean leaf wrinkling disease, the base sequence of the gene is shown as SEQ ID NO.1, the gene can be stably expressed in a leaf wrinkling environment and is slightly influenced by other adverse conditions, and a molecular marker primer group developed based on the molecular marker gene has the advantages that the gene can be used for identifying the southern soybean leaf wrinkling disease; according to the present invention, whether the leaf wrinkling type of the to-be-detected plant is the southern soybean leaf wrinkling disease can be rapidly and simply determined through the real-time fluorescent quantitative PCR experiment, and compared with the existing field identification method, the detection method has characteristics of short identification period, completion within one day at the soonest, and direct determination according to the real-time fluorescent quantitative PCR delta Ct value, and the difficulty of form distinguishing |
| format | Patent |
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according to the present invention, whether the leaf wrinkling type of the to-be-detected plant is the southern soybean leaf wrinkling disease can be rapidly and simply determined through the real-time fluorescent quantitative PCR experiment, and compared with the existing field identification method, the detection method has characteristics of short identification period, completion within one day at the soonest, and direct determination according to the real-time fluorescent quantitative PCR delta Ct value, and the difficulty of form distinguishing</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjjsOwjAQBdNQIOAOSw9FgihSoohPRUUfLfaarOJPtLYF6Tg6EeIAVPOK0dPMi_eZPIEJAphfbBllBNbkExtWmDh4CAZiyKkj8dMY74QeLKGBp7DvLfsHaI6EkTbggiWVLQo4lJ4EBmE34SEhD4Beg6ZE6vvrKHVBL4uZQRtp9eOiWJ-Ot-aypSG0FAdUU2Bqm2tZ7uu6qurysPvH-QCn5koH</recordid><startdate>20230421</startdate><enddate>20230421</enddate><creator>LIANG JIANG</creator><creator>GUO XIAOHONG</creator><creator>TANG FUYUE</creator><creator>CHEN WENJIE</creator><creator>NING DEJIAO</creator><creator>WEI QINGYUAN</creator><creator>CHEN YUAN</creator><scope>EVB</scope></search><sort><creationdate>20230421</creationdate><title>Gene for auxiliary identification of southern soybean leaf wrinkling disease, molecular marker primer group and detection method</title><author>LIANG JIANG ; 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according to the present invention, whether the leaf wrinkling type of the to-be-detected plant is the southern soybean leaf wrinkling disease can be rapidly and simply determined through the real-time fluorescent quantitative PCR experiment, and compared with the existing field identification method, the detection method has characteristics of short identification period, completion within one day at the soonest, and direct determination according to the real-time fluorescent quantitative PCR delta Ct value, and the difficulty of form distinguishing</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Gene for auxiliary identification of southern soybean leaf wrinkling disease, molecular marker primer group and detection method |
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