Gene for auxiliary identification of southern soybean leaf wrinkling disease, molecular marker primer group and detection method
The invention discloses a gene for auxiliary identification of southern soybean leaf wrinkling disease, the base sequence of the gene is shown as SEQ ID NO.1, the gene can be stably expressed in a leaf wrinkling environment and is slightly influenced by other adverse conditions, and a molecular mark...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a gene for auxiliary identification of southern soybean leaf wrinkling disease, the base sequence of the gene is shown as SEQ ID NO.1, the gene can be stably expressed in a leaf wrinkling environment and is slightly influenced by other adverse conditions, and a molecular marker primer group developed based on the molecular marker gene has the advantages that the gene can be used for identifying the southern soybean leaf wrinkling disease; according to the present invention, whether the leaf wrinkling type of the to-be-detected plant is the southern soybean leaf wrinkling disease can be rapidly and simply determined through the real-time fluorescent quantitative PCR experiment, and compared with the existing field identification method, the detection method has characteristics of short identification period, completion within one day at the soonest, and direct determination according to the real-time fluorescent quantitative PCR delta Ct value, and the difficulty of form distinguishing |
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