Detection chip as well as preparation method and sample injection method thereof

The detection chip is divided into at least one functional area (1), the functional area (1) comprises a reaction area (101) and a non-reaction area (102) surrounding the reaction area (101), and the detection chip comprises a first substrate (9) and a second substrate (10) which are oppositely arranged, a plurality of reaction tanks (4) which are arranged in an array along a first direction (X) and a second direction (Y) are arranged on one side, facing the second substrate (10), of the first substrate (9) and are positioned in the reaction area (101); a first circulating groove (5) connected with the two adjacent reaction grooves (4) is arranged between the two adjacent reaction grooves (4) in the first direction (X), and the first circulating groove (5) extends along the first direction (X); a second circulating groove (6) connected with the two first circulating grooves (5) is formed between the two adjacent first circulating grooves (5) in the second direction (Y), and the second circulating grooves (6)