Sperm DNA extraction kit and extraction method

The invention discloses a sperm DNA extraction kit and an extraction method, and belongs to the technical field of molecular biology. Wherein the extraction kit comprises a lysis solution, and the lysis solution comprises 0.9 to 2.5 percent of SDS (sodium dodecyl sulfate), 0.156 to 0.267 M of DTT (dithiothreitol), 0.19 to 0.21 mg/mL of protease and 5 to 12 ng/mu L of RNase A. The sperm DNA extracted by utilizing the kit and the method disclosed by the invention is high in total amount of the obtained DNA, complete in agarose gel detection main band and high in purity, and can be used for researching the hereditary characteristics of sperm source species and providing technical support for molecular breeding. 本发明公开了一种精子DNA提取试剂盒及提取方法，属于分子生物学技术领域。其中，所述提取试剂盒包括裂解液，所述裂解液中包括0.9～2.5％的SDS、0.156～0.267M的DTT，0.19～0.21mg/mL的蛋白酶和5～12ng/μL的RNase A。利用本发明的试剂盒和方法提取精子DNA，得到的DNA总量高、琼脂糖凝胶检测主条带完整、纯度高，能够用于研究精子来源物种的遗传特性，为分子育种提供技术支持。