Expression protein for screening toxic metals combined with and/or activating EGFR and preparation method thereof

The invention relates to the technical field of biology, particularly relates to the technical field of biology, and particularly relates to an expression protein for screening toxic metals combined with and/or activating EGFR (epidermal growth factor receptor) and a preparation method. The nucleotide sequence of cDNA (complementary deoxyribonucleic acid) of the expression protein is shown as SEQ ID NO. 1; the method comprises the following steps: introducing the vector into a host thallus to obtain a recombinant thallus; culturing the recombinant thalli, inducing protein expression, extracting and purifying, and performing enzyme digestion to obtain expressed protein; a plurality of free cysteine residue polypeptide peptide fragments close to a functional region are selected in a non-functional region of EGFR, so that cDNA fragments of expression protein are obtained, the cysteine residues in the expression protein are combined with toxic metals, and then the toxic metals are screened by using the combinatio