Coding gene and preparation method of recombinant porcine alpha interferon/interleukin 2 fusion protein

The invention relates to the technical field of gene engineering, in particular to a coding gene and a preparation method of a recombinant porcine alpha interferon/interleukin 2 fusion protein. The invention provides a coding gene of a recombinant porcine alpha interferon/interleukin 2 fusion protei...

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Hauptverfasser: LIU MIN, YAN RUOQIAN, MA ZHENYUAN, LIU MEIFEN, LIU YING, BAN FUGUO, XIE CAIHUA, SHIBA SHIGERU, ZHAO XUELI, FENG GUIDAN, GUO YUPEI, WANG DONGFANG, WANG CUI, YANG HAIBO, WANG SHUJUAN, WANG HUAJUN, ZHU QIANLEI
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creator LIU MIN
YAN RUOQIAN
MA ZHENYUAN
LIU MEIFEN
LIU YING
BAN FUGUO
XIE CAIHUA
SHIBA SHIGERU
ZHAO XUELI
FENG GUIDAN
GUO YUPEI
WANG DONGFANG
WANG CUI
YANG HAIBO
WANG SHUJUAN
WANG HUAJUN
ZHU QIANLEI
description The invention relates to the technical field of gene engineering, in particular to a coding gene and a preparation method of a recombinant porcine alpha interferon/interleukin 2 fusion protein. The invention provides a coding gene of a recombinant porcine alpha interferon/interleukin 2 fusion protein. The nucleotide sequence of the coding gene is shown as SEQ ID NO.1. The invention also provides a preparation method of the recombinant porcine alpha interferon/interleukin 2 fusion protein. The coding gene of the recombinant porcine interferon-alpha/interleukin 2 fusion protein can realize efficient and soluble expression in escherichia coli, and the soluble recombinant porcine interferon-alpha/interleukin 2 fusion protein is prepared; the purified recombinant porcine alpha interferon/interleukin 2 fusion protein has high biological activity on different cell lines, the virus proliferation inhibition activity of the recombinant porcine alpha interferon/interleukin 2 fusion protein is remarkably higher than that
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title Coding gene and preparation method of recombinant porcine alpha interferon/interleukin 2 fusion protein
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