Coding gene and preparation method of recombinant porcine alpha interferon/interleukin 2 fusion protein
The invention relates to the technical field of gene engineering, in particular to a coding gene and a preparation method of a recombinant porcine alpha interferon/interleukin 2 fusion protein. The invention provides a coding gene of a recombinant porcine alpha interferon/interleukin 2 fusion protei...
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creator | LIU MIN YAN RUOQIAN MA ZHENYUAN LIU MEIFEN LIU YING BAN FUGUO XIE CAIHUA SHIBA SHIGERU ZHAO XUELI FENG GUIDAN GUO YUPEI WANG DONGFANG WANG CUI YANG HAIBO WANG SHUJUAN WANG HUAJUN ZHU QIANLEI |
description | The invention relates to the technical field of gene engineering, in particular to a coding gene and a preparation method of a recombinant porcine alpha interferon/interleukin 2 fusion protein. The invention provides a coding gene of a recombinant porcine alpha interferon/interleukin 2 fusion protein. The nucleotide sequence of the coding gene is shown as SEQ ID NO.1. The invention also provides a preparation method of the recombinant porcine alpha interferon/interleukin 2 fusion protein. The coding gene of the recombinant porcine interferon-alpha/interleukin 2 fusion protein can realize efficient and soluble expression in escherichia coli, and the soluble recombinant porcine interferon-alpha/interleukin 2 fusion protein is prepared; the purified recombinant porcine alpha interferon/interleukin 2 fusion protein has high biological activity on different cell lines, the virus proliferation inhibition activity of the recombinant porcine alpha interferon/interleukin 2 fusion protein is remarkably higher than that |
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The invention provides a coding gene of a recombinant porcine alpha interferon/interleukin 2 fusion protein. The nucleotide sequence of the coding gene is shown as SEQ ID NO.1. The invention also provides a preparation method of the recombinant porcine alpha interferon/interleukin 2 fusion protein. The coding gene of the recombinant porcine interferon-alpha/interleukin 2 fusion protein can realize efficient and soluble expression in escherichia coli, and the soluble recombinant porcine interferon-alpha/interleukin 2 fusion protein is prepared; the purified recombinant porcine alpha interferon/interleukin 2 fusion protein has high biological activity on different cell lines, the virus proliferation inhibition activity of the recombinant porcine alpha interferon/interleukin 2 fusion protein is remarkably higher than that</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230407&DB=EPODOC&CC=CN&NR=115927415A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25562,76317</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230407&DB=EPODOC&CC=CN&NR=115927415A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LIU MIN</creatorcontrib><creatorcontrib>YAN RUOQIAN</creatorcontrib><creatorcontrib>MA ZHENYUAN</creatorcontrib><creatorcontrib>LIU MEIFEN</creatorcontrib><creatorcontrib>LIU YING</creatorcontrib><creatorcontrib>BAN FUGUO</creatorcontrib><creatorcontrib>XIE CAIHUA</creatorcontrib><creatorcontrib>SHIBA SHIGERU</creatorcontrib><creatorcontrib>ZHAO XUELI</creatorcontrib><creatorcontrib>FENG GUIDAN</creatorcontrib><creatorcontrib>GUO YUPEI</creatorcontrib><creatorcontrib>WANG DONGFANG</creatorcontrib><creatorcontrib>WANG CUI</creatorcontrib><creatorcontrib>YANG HAIBO</creatorcontrib><creatorcontrib>WANG SHUJUAN</creatorcontrib><creatorcontrib>WANG HUAJUN</creatorcontrib><creatorcontrib>ZHU QIANLEI</creatorcontrib><title>Coding gene and preparation method of recombinant porcine alpha interferon/interleukin 2 fusion protein</title><description>The invention relates to the technical field of gene engineering, in particular to a coding gene and a preparation method of a recombinant porcine alpha interferon/interleukin 2 fusion protein. 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The invention provides a coding gene of a recombinant porcine alpha interferon/interleukin 2 fusion protein. The nucleotide sequence of the coding gene is shown as SEQ ID NO.1. The invention also provides a preparation method of the recombinant porcine alpha interferon/interleukin 2 fusion protein. The coding gene of the recombinant porcine interferon-alpha/interleukin 2 fusion protein can realize efficient and soluble expression in escherichia coli, and the soluble recombinant porcine interferon-alpha/interleukin 2 fusion protein is prepared; the purified recombinant porcine alpha interferon/interleukin 2 fusion protein has high biological activity on different cell lines, the virus proliferation inhibition activity of the recombinant porcine alpha interferon/interleukin 2 fusion protein is remarkably higher than that</abstract><oa>free_for_read</oa></addata></record> |
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subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
title | Coding gene and preparation method of recombinant porcine alpha interferon/interleukin 2 fusion protein |
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