Coding gene and preparation method of recombinant porcine alpha interferon/interleukin 2 fusion protein
The invention relates to the technical field of gene engineering, in particular to a coding gene and a preparation method of a recombinant porcine alpha interferon/interleukin 2 fusion protein. The invention provides a coding gene of a recombinant porcine alpha interferon/interleukin 2 fusion protei...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention relates to the technical field of gene engineering, in particular to a coding gene and a preparation method of a recombinant porcine alpha interferon/interleukin 2 fusion protein. The invention provides a coding gene of a recombinant porcine alpha interferon/interleukin 2 fusion protein. The nucleotide sequence of the coding gene is shown as SEQ ID NO.1. The invention also provides a preparation method of the recombinant porcine alpha interferon/interleukin 2 fusion protein. The coding gene of the recombinant porcine interferon-alpha/interleukin 2 fusion protein can realize efficient and soluble expression in escherichia coli, and the soluble recombinant porcine interferon-alpha/interleukin 2 fusion protein is prepared; the purified recombinant porcine alpha interferon/interleukin 2 fusion protein has high biological activity on different cell lines, the virus proliferation inhibition activity of the recombinant porcine alpha interferon/interleukin 2 fusion protein is remarkably higher than that |
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