Coding gene and preparation method of recombinant porcine alpha interferon/interleukin 2 fusion protein

The invention relates to the technical field of gene engineering, in particular to a coding gene and a preparation method of a recombinant porcine alpha interferon/interleukin 2 fusion protein. The invention provides a coding gene of a recombinant porcine alpha interferon/interleukin 2 fusion protei...

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Hauptverfasser: LIU MIN, YAN RUOQIAN, MA ZHENYUAN, LIU MEIFEN, LIU YING, BAN FUGUO, XIE CAIHUA, SHIBA SHIGERU, ZHAO XUELI, FENG GUIDAN, GUO YUPEI, WANG DONGFANG, WANG CUI, YANG HAIBO, WANG SHUJUAN, WANG HUAJUN, ZHU QIANLEI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to the technical field of gene engineering, in particular to a coding gene and a preparation method of a recombinant porcine alpha interferon/interleukin 2 fusion protein. The invention provides a coding gene of a recombinant porcine alpha interferon/interleukin 2 fusion protein. The nucleotide sequence of the coding gene is shown as SEQ ID NO.1. The invention also provides a preparation method of the recombinant porcine alpha interferon/interleukin 2 fusion protein. The coding gene of the recombinant porcine interferon-alpha/interleukin 2 fusion protein can realize efficient and soluble expression in escherichia coli, and the soluble recombinant porcine interferon-alpha/interleukin 2 fusion protein is prepared; the purified recombinant porcine alpha interferon/interleukin 2 fusion protein has high biological activity on different cell lines, the virus proliferation inhibition activity of the recombinant porcine alpha interferon/interleukin 2 fusion protein is remarkably higher than that