Quantitative detection method of hyperoside and application

The invention is suitable for the technical field of drug detection, provides a quantitative detection method of hyperoside and application, and aims to obtain the exclusive content determination and detection method of hyperoside in Jianer Qingqing liquid, which has the advantages of simplicity, rapidness, good separation effect, high column efficiency, good durability, excellent reproducibility and the like, by optimizing chromatographic conditions. On one hand, a single-wavelength detection method is used, a standard variable wavelength ultraviolet detector can be used in a laboratory for execution, the method is simple and easy to implement, and the instrument configuration cost is reduced; on the other hand, the chromatographic separation efficiency is improved by optimizing the filler particle size, the mobile phase type, the mobile phase elution procedure and the like of the chromatographic column, and a sharper peak shape and a higher theoretical plate number are obtained; on the other hand, an ethyl