Method and kit for detecting adeno-associated virus vector infection titer
The invention discloses a method and a kit for detecting the infection titer of an adeno-associated virus vector, and relates to a method for detecting the infection titer of a recombinant adeno-associated virus vector sample. The method is characterized by comprising the following steps: taking a r...
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| creator | WANG CONG LI XIU YU SHUANGQING DONG XIAOYAN DONG ZHEYUE |
| description | The invention discloses a method and a kit for detecting the infection titer of an adeno-associated virus vector, and relates to a method for detecting the infection titer of a recombinant adeno-associated virus vector sample. The method is characterized by comprising the following steps: taking a recombinant adeno-associated virus vector sample and a recombinant HSV1 virus carrying a rep gene and a cap gene of AAV as auxiliary viruses to be in contact with cells sensitive to the HSV1 virus and the AAV virus, and culturing the cells; probes and primers are designed according to an AAV vector genome DNA sequence of a recombinant adeno-associated virus vector sample, quantitative PCR detection is carried out on the copy number of AAV genome DNA generated by replication and proliferation of the recombinant adeno-associated virus vector sample in cells, and therefore the infection titer of the recombinant adeno-associated virus vector sample is calculated. The invention also relates to a kit for implementing the |
| format | Patent |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | Method and kit for detecting adeno-associated virus vector infection titer |
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