Glycosylated cellobiose dehydrogenase, lactose biosensor and application thereof
The invention relates to the technical field of biological enzyme gene engineering and biosensing, in particular to a deglycosylated cellobiose dehydrogenase and lactose biosensor and application thereof. According to the invention, the cellobiose dehydrogenase from Neurospora crassa is subjected to...
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| creator | ZHANG ZHENYU ZHU SIRONG MENG QINGJUN MA YAOHONG GONG WEILI YANG YAN WANG BINGLIAN LIU QING'AI |
| description | The invention relates to the technical field of biological enzyme gene engineering and biosensing, in particular to a deglycosylated cellobiose dehydrogenase and lactose biosensor and application thereof. According to the invention, the cellobiose dehydrogenase from Neurospora crassa is subjected to codon optimization, so that the cellobiose dehydrogenase is smoothly expressed in pichia pastoris, purified protein is successfully obtained, the cellobiose dehydrogenase subjected to glycosylation removal is successfully obtained after the cellobiose dehydrogenase is treated by endonuclease Hf and mannosidase, and the cellobiose dehydrogenase subjected to glycosylation removal is obtained. And the lactose biosensor element capable of direct electron transfer is successfully prepared to be used for lactose detection, so that the rapid development of the lactose biosensor is effectively promoted, and therefore, the lactose biosensor element has a good practical application value.
本发明涉及生物酶基因工程和生物传感技术领域，具体涉及一种去糖基化的纤维 |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Glycosylated cellobiose dehydrogenase, lactose biosensor and application thereof |
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