Primer, kit and method for identifying capsicum annuum without lateral branches
The invention relates to a primer for identifying chilies without lateral branches. The primer comprises an upstream primer (5 '-3') and a downstream primer (5 '-3'), wherein the upstream primer (5 '-3') is TGTAAACGACGGCCAGTACATGCCTCTGACTGCTCT; and the downstream primer...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention relates to a primer for identifying chilies without lateral branches. The primer comprises an upstream primer (5 '-3') and a downstream primer (5 '-3'), wherein the upstream primer (5 '-3') is TGTAAACGACGGCCAGTACATGCCTCTGACTGCTCT; and the downstream primer (5 '-3') is AGCTGCTCCAGAACCAAAAA. The invention also relates to a kit for identifying the chilli without lateral branches and a method for identifying the chilli without lateral branches. The primer amplifies a 261bp band in single-stem pepper, has no band in clustered pod pepper, can perform molecular marker identification on pepper without lateral branches and branch pepper in the seed or seedling stage, saves manpower and material resources in the pepper breeding process, shortens the breeding time, and improves the accuracy and efficiency about whether branches exist or not in the breeding process.
本发明涉及一种鉴定无侧枝辣椒的引物。引物包括:上游引物(5'-3'):TGTAAAACGACGGCCAGTACATGCCCTCTGACTGCTCT;下游引物(5'-3'):AGCTGCTCCAGAACCAAAAA。本发明还涉及一种鉴定无侧枝辣椒的试剂盒以及鉴定无侧枝辣椒的方法。引物在独 |
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