Preparation of mesoporous silica microcapsule for immobilization optimization based on steric hindrance

The invention discloses preparation of a mesoporous silica microcapsule for immobilized optimization based on steric hindrance, and belongs to the technical field of capsules. The preparation method comprises the following steps: firstly, immobilizing glucose oxidase and catalase by using mesoporous silica, then forming a layer of polymer network film with good permeability on the surface of the mesoporous silica immobilized with double enzymes through room-temperature polymerization, and finally forming the electropositive enzyme compound nanocapsule Polymer (at) Protein (at) SiO2. The method not only ensures the stability of the enzyme, but also maintains the efficient catalytic activity of the enzyme due to the microenvironment of the APM node group on the polymer, and has higher enzyme retention efficiency and stress resistance compared with the single mesoporous silica physical adsorption. 基于空间位阻进行固定化优化的介孔二氧化硅微胶囊的制备，属于胶囊技术领域。第一步利用介孔二氧化硅固定葡萄糖氧化酶与过氧化氢酶，随后通过室温聚合在已固定双酶的介孔二氧化硅表面形成一层具有良好渗透性的聚合物网络膜，最终形成带正电酶复合物纳