Method for rapidly screening fish scale albumen glue tyrosinase inhibitory peptide at high throughput
The invention discloses a method for rapidly screening fish scale albumen glue tyrosinase inhibitory peptides with high throughput. The method comprises the following steps: S1, extracting fish scale albumen glue; s2, preparing a protein glue hydrolysate; s3, determining the optimal reaction concent...
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| creator | TU ZONGCAI LIU JUN YE YUNHUA HU ZIZI LI JINLIN WANG HUI SHA XIAOMEI |
| description | The invention discloses a method for rapidly screening fish scale albumen glue tyrosinase inhibitory peptides with high throughput. The method comprises the following steps: S1, extracting fish scale albumen glue; s2, preparing a protein glue hydrolysate; s3, determining the optimal reaction concentration of hydrolysate and tyrosinase; s4, carrying out biological affinity ultrafiltration; and S5, carrying out liquid chromatography-mass spectrometry combined high-throughput identification. Through the structure, the tyrosinase inhibitory peptide is screened by combining biological affinity ultrafiltration with a mass spectrometry technology, so that the separation and purification steps and the dosage of enzymes and samples in the screening process are reduced, the preparation efficiency is improved, and the bioactive polypeptide with tyrosinase inhibitory activity is prepared by taking a processing byproduct fish scale as a raw material. The high-value utilization of aquatic product processing byproducts is p |
| format | Patent |
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The method comprises the following steps: S1, extracting fish scale albumen glue; s2, preparing a protein glue hydrolysate; s3, determining the optimal reaction concentration of hydrolysate and tyrosinase; s4, carrying out biological affinity ultrafiltration; and S5, carrying out liquid chromatography-mass spectrometry combined high-throughput identification. Through the structure, the tyrosinase inhibitory peptide is screened by combining biological affinity ultrafiltration with a mass spectrometry technology, so that the separation and purification steps and the dosage of enzymes and samples in the screening process are reduced, the preparation efficiency is improved, and the bioactive polypeptide with tyrosinase inhibitory activity is prepared by taking a processing byproduct fish scale as a raw material. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for rapidly screening fish scale albumen glue tyrosinase inhibitory peptide at high throughput |
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