Method for evaluating genome composition of plant hybrid based on genome characteristics of trisomes
The invention provides a method for evaluating genome composition of plant hybrids based on genome characteristics of trisomes, and relates to the technical field of plant molecular biology. The method comprises the following steps: respectively carrying out flow cytometry on a plant hybrid and two...
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| creator | HE ZHENGSHAN JIA YANXIA YANG JUNBO ZENG CHUNXIA YANG ZHIYUN |
| description | The invention provides a method for evaluating genome composition of plant hybrids based on genome characteristics of trisomes, and relates to the technical field of plant molecular biology. The method comprises the following steps: respectively carrying out flow cytometry on a plant hybrid and two parents thereof to measure the sizes of genomes, respectively carrying out genome surface sequencing to obtain more than 30 * second-generation sequencing data, and finally carrying out k-mer analysis, nQuire analysis and Sudgeplot analysis on the sequencing data. And comprehensively comparing a plurality of results to evaluate a plurality of genome composition information such as genome size, ploidy, heterozygosis rate, repeatability and the like of the plant hybrids. The method is different from an existing plant genome characteristic evaluation method, the triome principle of a genome assembly species is fully used for reference, and after two parents of the plant hybrid are added, the genome composition of the |
| format | Patent |
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The method comprises the following steps: respectively carrying out flow cytometry on a plant hybrid and two parents thereof to measure the sizes of genomes, respectively carrying out genome surface sequencing to obtain more than 30 * second-generation sequencing data, and finally carrying out k-mer analysis, nQuire analysis and Sudgeplot analysis on the sequencing data. And comprehensively comparing a plurality of results to evaluate a plurality of genome composition information such as genome size, ploidy, heterozygosis rate, repeatability and the like of the plant hybrids. 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The method comprises the following steps: respectively carrying out flow cytometry on a plant hybrid and two parents thereof to measure the sizes of genomes, respectively carrying out genome surface sequencing to obtain more than 30 * second-generation sequencing data, and finally carrying out k-mer analysis, nQuire analysis and Sudgeplot analysis on the sequencing data. And comprehensively comparing a plurality of results to evaluate a plurality of genome composition information such as genome size, ploidy, heterozygosis rate, repeatability and the like of the plant hybrids. 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The method comprises the following steps: respectively carrying out flow cytometry on a plant hybrid and two parents thereof to measure the sizes of genomes, respectively carrying out genome surface sequencing to obtain more than 30 * second-generation sequencing data, and finally carrying out k-mer analysis, nQuire analysis and Sudgeplot analysis on the sequencing data. And comprehensively comparing a plurality of results to evaluate a plurality of genome composition information such as genome size, ploidy, heterozygosis rate, repeatability and the like of the plant hybrids. The method is different from an existing plant genome characteristic evaluation method, the triome principle of a genome assembly species is fully used for reference, and after two parents of the plant hybrid are added, the genome composition of the</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE |
| title | Method for evaluating genome composition of plant hybrid based on genome characteristics of trisomes |
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