Method for determining titer of gentamicin sulfate fermentation liquor
The invention provides a method for measuring the titer of a gentamicin sulfate fermentation broth, which comprises the following steps: 1) taking the gentamicin sulfate fermentation broth, and acidifying the gentamicin sulfate fermentation broth with concentrated sulfuric acid until the pH value is...
Gespeichert in:
| Hauptverfasser: | , , , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | ZHENG ZHAOXIA TANG ZHIJING MA SHAOHUA GE CUIFENG QIAO YANG |
| description | The invention provides a method for measuring the titer of a gentamicin sulfate fermentation broth, which comprises the following steps: 1) taking the gentamicin sulfate fermentation broth, and acidifying the gentamicin sulfate fermentation broth with concentrated sulfuric acid until the pH value is 1.5-2.0; 2) filtering, and neutralizing the filtrate with a sodium hydroxide solution until the pH value is 7.3 +/-0.5; (3) measuring the neutralized solution, putting into a centrifugal tube, adding a reyd's salt solution, and then centrifuging; 4) washing, precipitating and centrifuging; (5) adding an acetone aqueous solution to dissolve the precipitate, centrifuging, taking supernate, and measuring absorbance by adopting an ultraviolet-visible spectrophotometric method; (6) weighing a gentamicin sulfate standard substance, and preparing supernate according to the same treatment method to obtain a standard curve and a linear regression equation of the relationship between the unit titer and the absorbance of the |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN115184288A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN115184288A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN115184288A3</originalsourceid><addsrcrecordid>eNrjZHDzTS3JyE9RSMsvUkhJLUktys3My8xLVyjJBLIV8tMU0lPzShJzM5Mz8xSKS3PSEktSFdKAqkCiJZn5eQo5mYWl-UU8DKxpiTnFqbxQmptB0c01xNlDN7UgPz61uCAxOTUvtSTe2c_Q0NTQwsTIwsLRmBg1AGlgNC8</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Method for determining titer of gentamicin sulfate fermentation liquor</title><source>esp@cenet</source><creator>ZHENG ZHAOXIA ; TANG ZHIJING ; MA SHAOHUA ; GE CUIFENG ; QIAO YANG</creator><creatorcontrib>ZHENG ZHAOXIA ; TANG ZHIJING ; MA SHAOHUA ; GE CUIFENG ; QIAO YANG</creatorcontrib><description>The invention provides a method for measuring the titer of a gentamicin sulfate fermentation broth, which comprises the following steps: 1) taking the gentamicin sulfate fermentation broth, and acidifying the gentamicin sulfate fermentation broth with concentrated sulfuric acid until the pH value is 1.5-2.0; 2) filtering, and neutralizing the filtrate with a sodium hydroxide solution until the pH value is 7.3 +/-0.5; (3) measuring the neutralized solution, putting into a centrifugal tube, adding a reyd's salt solution, and then centrifuging; 4) washing, precipitating and centrifuging; (5) adding an acetone aqueous solution to dissolve the precipitate, centrifuging, taking supernate, and measuring absorbance by adopting an ultraviolet-visible spectrophotometric method; (6) weighing a gentamicin sulfate standard substance, and preparing supernate according to the same treatment method to obtain a standard curve and a linear regression equation of the relationship between the unit titer and the absorbance of the</description><language>chi ; eng</language><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; PHYSICS ; TESTING</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20221014&DB=EPODOC&CC=CN&NR=115184288A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76294</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20221014&DB=EPODOC&CC=CN&NR=115184288A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ZHENG ZHAOXIA</creatorcontrib><creatorcontrib>TANG ZHIJING</creatorcontrib><creatorcontrib>MA SHAOHUA</creatorcontrib><creatorcontrib>GE CUIFENG</creatorcontrib><creatorcontrib>QIAO YANG</creatorcontrib><title>Method for determining titer of gentamicin sulfate fermentation liquor</title><description>The invention provides a method for measuring the titer of a gentamicin sulfate fermentation broth, which comprises the following steps: 1) taking the gentamicin sulfate fermentation broth, and acidifying the gentamicin sulfate fermentation broth with concentrated sulfuric acid until the pH value is 1.5-2.0; 2) filtering, and neutralizing the filtrate with a sodium hydroxide solution until the pH value is 7.3 +/-0.5; (3) measuring the neutralized solution, putting into a centrifugal tube, adding a reyd's salt solution, and then centrifuging; 4) washing, precipitating and centrifuging; (5) adding an acetone aqueous solution to dissolve the precipitate, centrifuging, taking supernate, and measuring absorbance by adopting an ultraviolet-visible spectrophotometric method; (6) weighing a gentamicin sulfate standard substance, and preparing supernate according to the same treatment method to obtain a standard curve and a linear regression equation of the relationship between the unit titer and the absorbance of the</description><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>PHYSICS</subject><subject>TESTING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZHDzTS3JyE9RSMsvUkhJLUktys3My8xLVyjJBLIV8tMU0lPzShJzM5Mz8xSKS3PSEktSFdKAqkCiJZn5eQo5mYWl-UU8DKxpiTnFqbxQmptB0c01xNlDN7UgPz61uCAxOTUvtSTe2c_Q0NTQwsTIwsLRmBg1AGlgNC8</recordid><startdate>20221014</startdate><enddate>20221014</enddate><creator>ZHENG ZHAOXIA</creator><creator>TANG ZHIJING</creator><creator>MA SHAOHUA</creator><creator>GE CUIFENG</creator><creator>QIAO YANG</creator><scope>EVB</scope></search><sort><creationdate>20221014</creationdate><title>Method for determining titer of gentamicin sulfate fermentation liquor</title><author>ZHENG ZHAOXIA ; TANG ZHIJING ; MA SHAOHUA ; GE CUIFENG ; QIAO YANG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN115184288A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2022</creationdate><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>PHYSICS</topic><topic>TESTING</topic><toplevel>online_resources</toplevel><creatorcontrib>ZHENG ZHAOXIA</creatorcontrib><creatorcontrib>TANG ZHIJING</creatorcontrib><creatorcontrib>MA SHAOHUA</creatorcontrib><creatorcontrib>GE CUIFENG</creatorcontrib><creatorcontrib>QIAO YANG</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>ZHENG ZHAOXIA</au><au>TANG ZHIJING</au><au>MA SHAOHUA</au><au>GE CUIFENG</au><au>QIAO YANG</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for determining titer of gentamicin sulfate fermentation liquor</title><date>2022-10-14</date><risdate>2022</risdate><abstract>The invention provides a method for measuring the titer of a gentamicin sulfate fermentation broth, which comprises the following steps: 1) taking the gentamicin sulfate fermentation broth, and acidifying the gentamicin sulfate fermentation broth with concentrated sulfuric acid until the pH value is 1.5-2.0; 2) filtering, and neutralizing the filtrate with a sodium hydroxide solution until the pH value is 7.3 +/-0.5; (3) measuring the neutralized solution, putting into a centrifugal tube, adding a reyd's salt solution, and then centrifuging; 4) washing, precipitating and centrifuging; (5) adding an acetone aqueous solution to dissolve the precipitate, centrifuging, taking supernate, and measuring absorbance by adopting an ultraviolet-visible spectrophotometric method; (6) weighing a gentamicin sulfate standard substance, and preparing supernate according to the same treatment method to obtain a standard curve and a linear regression equation of the relationship between the unit titer and the absorbance of the</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | chi ; eng |
| recordid | cdi_epo_espacenet_CN115184288A |
| source | esp@cenet |
| subjects | INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PHYSICS TESTING |
| title | Method for determining titer of gentamicin sulfate fermentation liquor |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T04%3A46%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=ZHENG%20ZHAOXIA&rft.date=2022-10-14&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN115184288A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |