Application of tannic acid in inhibiting polyspermic fertilization of porcine oocytes

The invention provides application of tannic acid in inhibiting polyspermic fertilization of porcine oocytes, and belongs to the technical field of animal husbandry. The method is used for effectively inhibiting polyspermia fertilization and guaranteeing the later-stage embryonic development quality. The porcine oocyte in-vitro maturation culture solution with the effect of inhibiting polyspermia fertilization is prepared from 0.6 mmol/L of cysteine, 0.91 mmol/L of sodium pyruvate, 10ng/mL of EGF, 10% of porcine follicular fluid, 10IU/mL of FSH, 10IU/mL of LH, 1% of mycillin and 1-10mu g/mL of tannic acid on the basis of TCM199. The pig oocyte body culture solution containing the tannic acid inhibits the polyspermia fertilization effect of oocytes in the in-vitro fertilization process, and the later embryo development quality is guaranteed. 本发明提供了单宁酸在抑制猪卵母细胞多精受精中的应用，属于畜牧技术领域。为了有效抑制多精受精的发生，保障后期胚胎发育质量。本发明的具有抑制多精受精作用的猪卵母细胞体外成熟培养液是以TCM199为基准计，0.6mmol/L半胱氨酸，0.91mmol/L丙酮酸钠，10ng/mL EGF，10％猪卵泡液，10IU/mL FSH，10IU/mL LH