Polypeptides comprising human VEGF-A mutants with disulfide bridge rearrangement and compositions comprising same

Polypeptides comprising a functional mutant of an isoform of human vascular endothelial growth factor A (VEGF-A) folded with a non-natural rearrangement of a disulfide bridge wherein the second and fourth cysteines of the polypeptide chain of the mutant form only an intramolecular bridge and the seventh and eighth cysteines of the mutant form only an intermolecular bridge. The invention also includes antigen preparations comprising at least one of these polypeptides, as well as pharmaceutical compositions comprising said antigen preparations and vaccine adjuvants. The antigen preparations of the invention are used in the preparation of medicaments for the treatment of diseases whose progression is associated with increased angiogenesis, inflammation and immunosuppression, and for the recovery of the immune system. 包含以二硫桥的非天然重排进行折叠的人血管内皮生长因子A(VEGF-A)的同种型的功能突变体的多肽，其中所述突变体的多肽链的第二个和第四个半胱氨酸仅形成分子内桥，并且所述突变体的第七个和第八个半胱氨酸仅形成分子间桥。本发明还包括包含这些多肽中的至少一种的抗原制备物，以及包含所述抗原制备物和疫苗佐剂的药学组合物。本发明的抗原制备物在制备药物中使用，所述药物用于治疗其进展与血管发生、炎症和免疫抑制的