Method for constructing recombinant vibrio natriureticus for producing geraniol, recombinant vibrio natriureticus constructed by method and application of recombinant vibrio natriureticus
The invention provides a method for constructing recombinant vibrio natriticus for producing geraniol, the recombinant vibrio natriticus constructed by the method and application of the recombinant vibrio natriticus. The recombinant vibrio natriureticus can be subjected to fermentation culture throu...
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | YANG BOQIN SHEN JINGYUN CHENG BINGJING |
| description | The invention provides a method for constructing recombinant vibrio natriticus for producing geraniol, the recombinant vibrio natriticus constructed by the method and application of the recombinant vibrio natriticus. The recombinant vibrio natriureticus can be subjected to fermentation culture through conventional vibrio natriureticus and an LBv2 culture medium, high-yield geraniol expression is obtained through IPTG induction in the culture process and is about 9.97 mg/L, the yield (1.22 mg/L) of geraniol is obviously increased compared with that of geraniol produced by using escherichia coli as chassis cells, and the recombinant vibrio natriureticus has high geraniol industrialization potential.
本发明提供了构建生产香叶醇的重组需钠弧菌的方法以及通过该方法构建的重组需钠弧菌及其应用。本发明的重组需钠弧菌能够通过常规的需钠弧菌和LBv2培养基即可进行发酵培养，培养过程中通过IPTG诱导，获得高产量的香叶醇表达，约9.97mg/L，比使用大肠杆菌作为底盘细胞产生的香叶醇产量(1.22mg/L)明显提高，具有较强的香叶醇工业化潜力。 |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN114990147A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN114990147A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN114990147A3</originalsourceid><addsrcrecordid>eNqNjr0KwkAQhNNYiPoOa69gMCApJSg2WtmHy2UTF5LdY-9O8Nl8OX-xjcUwzXwzM07uRwwXqaERBSvsg0YbiFtQtNJXxIYDXKlSEmATlKJiIBv9m3AqdbSveItqmKRbDIO_GayhukH_OWD4Kec6siaQMEgz2DRNRo3pPM6-Pknm-925OCzRSYneGYuMoSxOaZrl-SrNNtv1P5kHo4RiNw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Method for constructing recombinant vibrio natriureticus for producing geraniol, recombinant vibrio natriureticus constructed by method and application of recombinant vibrio natriureticus</title><source>esp@cenet</source><creator>YANG BOQIN ; SHEN JINGYUN ; CHENG BINGJING</creator><creatorcontrib>YANG BOQIN ; SHEN JINGYUN ; CHENG BINGJING</creatorcontrib><description>The invention provides a method for constructing recombinant vibrio natriticus for producing geraniol, the recombinant vibrio natriticus constructed by the method and application of the recombinant vibrio natriticus. The recombinant vibrio natriureticus can be subjected to fermentation culture through conventional vibrio natriureticus and an LBv2 culture medium, high-yield geraniol expression is obtained through IPTG induction in the culture process and is about 9.97 mg/L, the yield (1.22 mg/L) of geraniol is obviously increased compared with that of geraniol produced by using escherichia coli as chassis cells, and the recombinant vibrio natriureticus has high geraniol industrialization potential.
本发明提供了构建生产香叶醇的重组需钠弧菌的方法以及通过该方法构建的重组需钠弧菌及其应用。本发明的重组需钠弧菌能够通过常规的需钠弧菌和LBv2培养基即可进行发酵培养，培养过程中通过IPTG诱导，获得高产量的香叶醇表达，约9.97mg/L，比使用大肠杆菌作为底盘细胞产生的香叶醇产量(1.22mg/L)明显提高，具有较强的香叶醇工业化潜力。</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220902&DB=EPODOC&CC=CN&NR=114990147A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220902&DB=EPODOC&CC=CN&NR=114990147A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>YANG BOQIN</creatorcontrib><creatorcontrib>SHEN JINGYUN</creatorcontrib><creatorcontrib>CHENG BINGJING</creatorcontrib><title>Method for constructing recombinant vibrio natriureticus for producing geraniol, recombinant vibrio natriureticus constructed by method and application of recombinant vibrio natriureticus</title><description>The invention provides a method for constructing recombinant vibrio natriticus for producing geraniol, the recombinant vibrio natriticus constructed by the method and application of the recombinant vibrio natriticus. The recombinant vibrio natriureticus can be subjected to fermentation culture through conventional vibrio natriureticus and an LBv2 culture medium, high-yield geraniol expression is obtained through IPTG induction in the culture process and is about 9.97 mg/L, the yield (1.22 mg/L) of geraniol is obviously increased compared with that of geraniol produced by using escherichia coli as chassis cells, and the recombinant vibrio natriureticus has high geraniol industrialization potential.
本发明提供了构建生产香叶醇的重组需钠弧菌的方法以及通过该方法构建的重组需钠弧菌及其应用。本发明的重组需钠弧菌能够通过常规的需钠弧菌和LBv2培养基即可进行发酵培养，培养过程中通过IPTG诱导，获得高产量的香叶醇表达，约9.97mg/L，比使用大肠杆菌作为底盘细胞产生的香叶醇产量(1.22mg/L)明显提高，具有较强的香叶醇工业化潜力。</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjr0KwkAQhNNYiPoOa69gMCApJSg2WtmHy2UTF5LdY-9O8Nl8OX-xjcUwzXwzM07uRwwXqaERBSvsg0YbiFtQtNJXxIYDXKlSEmATlKJiIBv9m3AqdbSveItqmKRbDIO_GayhukH_OWD4Kec6siaQMEgz2DRNRo3pPM6-Pknm-925OCzRSYneGYuMoSxOaZrl-SrNNtv1P5kHo4RiNw</recordid><startdate>20220902</startdate><enddate>20220902</enddate><creator>YANG BOQIN</creator><creator>SHEN JINGYUN</creator><creator>CHENG BINGJING</creator><scope>EVB</scope></search><sort><creationdate>20220902</creationdate><title>Method for constructing recombinant vibrio natriureticus for producing geraniol, recombinant vibrio natriureticus constructed by method and application of recombinant vibrio natriureticus</title><author>YANG BOQIN ; SHEN JINGYUN ; CHENG BINGJING</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN114990147A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2022</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>YANG BOQIN</creatorcontrib><creatorcontrib>SHEN JINGYUN</creatorcontrib><creatorcontrib>CHENG BINGJING</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>YANG BOQIN</au><au>SHEN JINGYUN</au><au>CHENG BINGJING</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for constructing recombinant vibrio natriureticus for producing geraniol, recombinant vibrio natriureticus constructed by method and application of recombinant vibrio natriureticus</title><date>2022-09-02</date><risdate>2022</risdate><abstract>The invention provides a method for constructing recombinant vibrio natriticus for producing geraniol, the recombinant vibrio natriticus constructed by the method and application of the recombinant vibrio natriticus. The recombinant vibrio natriureticus can be subjected to fermentation culture through conventional vibrio natriureticus and an LBv2 culture medium, high-yield geraniol expression is obtained through IPTG induction in the culture process and is about 9.97 mg/L, the yield (1.22 mg/L) of geraniol is obviously increased compared with that of geraniol produced by using escherichia coli as chassis cells, and the recombinant vibrio natriureticus has high geraniol industrialization potential.
本发明提供了构建生产香叶醇的重组需钠弧菌的方法以及通过该方法构建的重组需钠弧菌及其应用。本发明的重组需钠弧菌能够通过常规的需钠弧菌和LBv2培养基即可进行发酵培养，培养过程中通过IPTG诱导，获得高产量的香叶醇表达，约9.97mg/L，比使用大肠杆菌作为底盘细胞产生的香叶醇产量(1.22mg/L)明显提高，具有较强的香叶醇工业化潜力。</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | chi ; eng |
| recordid | cdi_epo_espacenet_CN114990147A |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for constructing recombinant vibrio natriureticus for producing geraniol, recombinant vibrio natriureticus constructed by method and application of recombinant vibrio natriureticus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T16%3A33%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=YANG%20BOQIN&rft.date=2022-09-02&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN114990147A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |