Construction and application of curvularia lunata steroid C14 alpha-hydroxylase CYP5103B5 mutant
The invention provides curvularia lunata steroid C14 alpha-hydroxylase mutants Y10 (S129A/T130A/D131G/T132L/V133I/R134K/S135K) and D131G, and the coding nucleotide sequences of the curvularia lunata steroid C14 alpha-hydroxylase mutants Y10 (S129A/T130A/D131G/T132L/V133I/R134K/S135K) and D131G are s...
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| creator | WANG SHUO MAO SHUHONG LIU XIAOGUANG QIAO JIANGYU LU FUPING |
| description | The invention provides curvularia lunata steroid C14 alpha-hydroxylase mutants Y10 (S129A/T130A/D131G/T132L/V133I/R134K/S135K) and D131G, and the coding nucleotide sequences of the curvularia lunata steroid C14 alpha-hydroxylase mutants Y10 (S129A/T130A/D131G/T132L/V133I/R134K/S135K) and D131G are shown as SEQ ID NO: 4 and SEQ ID NO: 6. Compared with C14 alpha-hydroxylase CYP5103B5, the mutant Y10 (S129A/T130A/D131G/T132L/V133I/R134K/S135K) and D131G have the advantages that the specificity of conversion of steroid substrate progesterone is obviously improved, and by-products are obviously reduced. The invention further provides an expression vector for heterologous overexpression of the CYP5103B5 mutant and a steroid transformation process of the expression vector, and valuable materials (genes and strains) and basic data support are provided for research and development of an efficient progesterone C14 alpha-hydroxylation production process.
本发明专利提供了新月弯孢霉甾体C14α-羟化酶突变体Y10(S129A/T130A/D131G/T132L/V133I/R134K/ |
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本发明专利提供了新月弯孢霉甾体C14α-羟化酶突变体Y10(S129A/T130A/D131G/T132L/V133I/R134K/</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220830&DB=EPODOC&CC=CN&NR=114958787A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,777,882,25545,76296</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220830&DB=EPODOC&CC=CN&NR=114958787A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>WANG SHUO</creatorcontrib><creatorcontrib>MAO SHUHONG</creatorcontrib><creatorcontrib>LIU XIAOGUANG</creatorcontrib><creatorcontrib>QIAO JIANGYU</creatorcontrib><creatorcontrib>LU FUPING</creatorcontrib><title>Construction and application of curvularia lunata steroid C14 alpha-hydroxylase CYP5103B5 mutant</title><description>The invention provides curvularia lunata steroid C14 alpha-hydroxylase mutants Y10 (S129A/T130A/D131G/T132L/V133I/R134K/S135K) and D131G, and the coding nucleotide sequences of the curvularia lunata steroid C14 alpha-hydroxylase mutants Y10 (S129A/T130A/D131G/T132L/V133I/R134K/S135K) and D131G are shown as SEQ ID NO: 4 and SEQ ID NO: 6. Compared with C14 alpha-hydroxylase CYP5103B5, the mutant Y10 (S129A/T130A/D131G/T132L/V133I/R134K/S135K) and D131G have the advantages that the specificity of conversion of steroid substrate progesterone is obviously improved, and by-products are obviously reduced. The invention further provides an expression vector for heterologous overexpression of the CYP5103B5 mutant and a steroid transformation process of the expression vector, and valuable materials (genes and strains) and basic data support are provided for research and development of an efficient progesterone C14 alpha-hydroxylation production process.
本发明专利提供了新月弯孢霉甾体C14α-羟化酶突变体Y10(S129A/T130A/D131G/T132L/V133I/R134K/</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyjEKwjAUgOEuDqLe4XmAgqEtbUcNipM4uDjVR_JKAzEJyYvY2wviAZx-PviXxUN6lzhmxcY7QKcBQ7BG4dd-BJXjK1uMBsFmh4yQmKI3GqSoAW2YsJxmHf17tpgI5P3aiF11aOCZGR2vi8WINtHm11WxPR1v8lxS8AOlgIoc8SAvQtR907Vdu6_-eT55hjxr</recordid><startdate>20220830</startdate><enddate>20220830</enddate><creator>WANG SHUO</creator><creator>MAO SHUHONG</creator><creator>LIU XIAOGUANG</creator><creator>QIAO JIANGYU</creator><creator>LU FUPING</creator><scope>EVB</scope></search><sort><creationdate>20220830</creationdate><title>Construction and application of curvularia lunata steroid C14 alpha-hydroxylase CYP5103B5 mutant</title><author>WANG SHUO ; MAO SHUHONG ; LIU XIAOGUANG ; QIAO JIANGYU ; LU FUPING</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN114958787A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2022</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>WANG SHUO</creatorcontrib><creatorcontrib>MAO SHUHONG</creatorcontrib><creatorcontrib>LIU XIAOGUANG</creatorcontrib><creatorcontrib>QIAO JIANGYU</creatorcontrib><creatorcontrib>LU FUPING</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>WANG SHUO</au><au>MAO SHUHONG</au><au>LIU XIAOGUANG</au><au>QIAO JIANGYU</au><au>LU FUPING</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Construction and application of curvularia lunata steroid C14 alpha-hydroxylase CYP5103B5 mutant</title><date>2022-08-30</date><risdate>2022</risdate><abstract>The invention provides curvularia lunata steroid C14 alpha-hydroxylase mutants Y10 (S129A/T130A/D131G/T132L/V133I/R134K/S135K) and D131G, and the coding nucleotide sequences of the curvularia lunata steroid C14 alpha-hydroxylase mutants Y10 (S129A/T130A/D131G/T132L/V133I/R134K/S135K) and D131G are shown as SEQ ID NO: 4 and SEQ ID NO: 6. Compared with C14 alpha-hydroxylase CYP5103B5, the mutant Y10 (S129A/T130A/D131G/T132L/V133I/R134K/S135K) and D131G have the advantages that the specificity of conversion of steroid substrate progesterone is obviously improved, and by-products are obviously reduced. The invention further provides an expression vector for heterologous overexpression of the CYP5103B5 mutant and a steroid transformation process of the expression vector, and valuable materials (genes and strains) and basic data support are provided for research and development of an efficient progesterone C14 alpha-hydroxylation production process.
本发明专利提供了新月弯孢霉甾体C14α-羟化酶突变体Y10(S129A/T130A/D131G/T132L/V133I/R134K/</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Construction and application of curvularia lunata steroid C14 alpha-hydroxylase CYP5103B5 mutant |
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