VIGS method for tea tree gene function research
The invention relates to a VIGS method for tea tree gene function research, which comprises the following steps: selecting tea tree seedlings, removing terminal buds and part of true leaves, and carrying out vacuum infection under the vacuum infection conditions that the infection pressure is 0.7-0....
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | LYU LITANG LI GUODONG YAO XINZHUAN |
| description | The invention relates to a VIGS method for tea tree gene function research, which comprises the following steps: selecting tea tree seedlings, removing terminal buds and part of true leaves, and carrying out vacuum infection under the vacuum infection conditions that the infection pressure is 0.7-0.8 kPa and the concentration D600 of bacterial liquid is 1.2 2O; the soaking time is 0.5 to 1 hour. The method provided by the invention shows that the tea tree VIGS method can silence the endogenous gene of the tea tree, research the gene function of the tea tree, make up the defect that the tea tree has no stable genetic transformation system, and lay a foundation for future research on the genome of the tea tree.
本发明涉及一种用于茶树基因功能研究的VIGS方法，选择茶树实生苗，去除顶芽和部分真叶，进行真空侵染，所述真空侵染条件为：侵染压强为0.7-0.8kPa、菌液浓度D600为1.2O；侵浸时间为0.5-1h。本发明的方法表明，茶树VIGS方法能够沉默茶树内源基因，研究茶树基因功能，弥补茶树没有稳定遗传转化体系的不足，为未来研究茶树基因组奠定了基础。 |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN114941009A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN114941009A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN114941009A3</originalsourceid><addsrcrecordid>eNrjZNAP83QPVshNLcnIT1FIyy9SKElNVCgpSk1VSE_NS1VIK81LLsnMz1MoSi1OTSxKzuBhYE1LzClO5YXS3AyKbq4hzh66qQX58anFBYnJQG0l8c5-hoYmliaGBgaWjsbEqAEAzvQqLQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>VIGS method for tea tree gene function research</title><source>esp@cenet</source><creator>LYU LITANG ; LI GUODONG ; YAO XINZHUAN</creator><creatorcontrib>LYU LITANG ; LI GUODONG ; YAO XINZHUAN</creatorcontrib><description>The invention relates to a VIGS method for tea tree gene function research, which comprises the following steps: selecting tea tree seedlings, removing terminal buds and part of true leaves, and carrying out vacuum infection under the vacuum infection conditions that the infection pressure is 0.7-0.8 kPa and the concentration D600 of bacterial liquid is 1.2 2O; the soaking time is 0.5 to 1 hour. The method provided by the invention shows that the tea tree VIGS method can silence the endogenous gene of the tea tree, research the gene function of the tea tree, make up the defect that the tea tree has no stable genetic transformation system, and lay a foundation for future research on the genome of the tea tree.
本发明涉及一种用于茶树基因功能研究的VIGS方法，选择茶树实生苗，去除顶芽和部分真叶，进行真空侵染，所述真空侵染条件为：侵染压强为0.7-0.8kPa、菌液浓度D600为1.2O；侵浸时间为0.5-1h。本发明的方法表明，茶树VIGS方法能够沉默茶树内源基因，研究茶树基因功能，弥补茶树没有稳定遗传转化体系的不足，为未来研究茶树基因组奠定了基础。</description><language>chi ; eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED ; CULTURE MEDIA ; ENZYMOLOGY ; FISHING ; FORESTRY ; HORTICULTURE ; HUMAN NECESSITIES ; HUNTING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; NEW PLANTS OR PROCESSES FOR OBTAINING THEM ; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TRAPPING ; VINEGAR ; WATERING ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220826&DB=EPODOC&CC=CN&NR=114941009A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220826&DB=EPODOC&CC=CN&NR=114941009A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LYU LITANG</creatorcontrib><creatorcontrib>LI GUODONG</creatorcontrib><creatorcontrib>YAO XINZHUAN</creatorcontrib><title>VIGS method for tea tree gene function research</title><description>The invention relates to a VIGS method for tea tree gene function research, which comprises the following steps: selecting tea tree seedlings, removing terminal buds and part of true leaves, and carrying out vacuum infection under the vacuum infection conditions that the infection pressure is 0.7-0.8 kPa and the concentration D600 of bacterial liquid is 1.2 2O; the soaking time is 0.5 to 1 hour. The method provided by the invention shows that the tea tree VIGS method can silence the endogenous gene of the tea tree, research the gene function of the tea tree, make up the defect that the tea tree has no stable genetic transformation system, and lay a foundation for future research on the genome of the tea tree.
本发明涉及一种用于茶树基因功能研究的VIGS方法，选择茶树实生苗，去除顶芽和部分真叶，进行真空侵染，所述真空侵染条件为：侵染压强为0.7-0.8kPa、菌液浓度D600为1.2O；侵浸时间为0.5-1h。本发明的方法表明，茶树VIGS方法能够沉默茶树内源基因，研究茶树基因功能，弥补茶树没有稳定遗传转化体系的不足，为未来研究茶树基因组奠定了基础。</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HORTICULTURE</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</subject><subject>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TRAPPING</subject><subject>VINEGAR</subject><subject>WATERING</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZNAP83QPVshNLcnIT1FIyy9SKElNVCgpSk1VSE_NS1VIK81LLsnMz1MoSi1OTSxKzuBhYE1LzClO5YXS3AyKbq4hzh66qQX58anFBYnJQG0l8c5-hoYmliaGBgaWjsbEqAEAzvQqLQ</recordid><startdate>20220826</startdate><enddate>20220826</enddate><creator>LYU LITANG</creator><creator>LI GUODONG</creator><creator>YAO XINZHUAN</creator><scope>EVB</scope></search><sort><creationdate>20220826</creationdate><title>VIGS method for tea tree gene function research</title><author>LYU LITANG ; LI GUODONG ; YAO XINZHUAN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN114941009A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2022</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HORTICULTURE</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</topic><topic>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TRAPPING</topic><topic>VINEGAR</topic><topic>WATERING</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LYU LITANG</creatorcontrib><creatorcontrib>LI GUODONG</creatorcontrib><creatorcontrib>YAO XINZHUAN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LYU LITANG</au><au>LI GUODONG</au><au>YAO XINZHUAN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>VIGS method for tea tree gene function research</title><date>2022-08-26</date><risdate>2022</risdate><abstract>The invention relates to a VIGS method for tea tree gene function research, which comprises the following steps: selecting tea tree seedlings, removing terminal buds and part of true leaves, and carrying out vacuum infection under the vacuum infection conditions that the infection pressure is 0.7-0.8 kPa and the concentration D600 of bacterial liquid is 1.2 2O; the soaking time is 0.5 to 1 hour. The method provided by the invention shows that the tea tree VIGS method can silence the endogenous gene of the tea tree, research the gene function of the tea tree, make up the defect that the tea tree has no stable genetic transformation system, and lay a foundation for future research on the genome of the tea tree.
本发明涉及一种用于茶树基因功能研究的VIGS方法，选择茶树实生苗，去除顶芽和部分真叶，进行真空侵染，所述真空侵染条件为：侵染压强为0.7-0.8kPa、菌液浓度D600为1.2O；侵浸时间为0.5-1h。本发明的方法表明，茶树VIGS方法能够沉默茶树内源基因，研究茶树基因功能，弥补茶树没有稳定遗传转化体系的不足，为未来研究茶树基因组奠定了基础。</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | chi ; eng |
| recordid | cdi_epo_espacenet_CN114941009A |
| source | esp@cenet |
| subjects | AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HORTICULTURE HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WATERING WINE |
| title | VIGS method for tea tree gene function research |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-24T16%3A18%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=LYU%20LITANG&rft.date=2022-08-26&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN114941009A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |