Engineering strain for producing cordycepin and cordycepin derivative 3 '-deoxyinosine as well as preparation method and application of engineering strain
The invention belongs to the field of genetic engineering and process engineering, and discloses an engineering strain for producing cordycepin and a cordycepin derivative 3 '-deoxyinosine as well as a preparation method and application of the engineering strain. Comprising codon optimized gene...
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| creator | TAN HUIPING FENG BAOMIN KAN TUO JIANG RONGSHUAI WEI DAPENG LI CHENYANG WANG LIANG LI XIANG LI QIAN LI XIUPING CHENG YANGHAO |
| description | The invention belongs to the field of genetic engineering and process engineering, and discloses an engineering strain for producing cordycepin and a cordycepin derivative 3 '-deoxyinosine as well as a preparation method and application of the engineering strain. Comprising codon optimized genes Cm1 and Cm2, a gene amplification and recovery fragment, a methanol induced promoter fragment and a terminator fragment, an expression vector is connected to the genes and then transferred into pichia pastoris for screening, and the pichia pastoris engineering strain protected by the invention is obtained and can be applied to production of cordycepin and derivatives thereof. The engineering strain prepared by the preparation method has huge advantages in production of cordycepin and derivatives thereof, the production efficiency of cordycepin is greatly improved due to the fact that the specific Cm1 and Cm2 genes are produced by utilizing the specific cordyceps militaris strain, and a green process for producing cord |
| format | Patent |
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Comprising codon optimized genes Cm1 and Cm2, a gene amplification and recovery fragment, a methanol induced promoter fragment and a terminator fragment, an expression vector is connected to the genes and then transferred into pichia pastoris for screening, and the pichia pastoris engineering strain protected by the invention is obtained and can be applied to production of cordycepin and derivatives thereof. The engineering strain prepared by the preparation method has huge advantages in production of cordycepin and derivatives thereof, the production efficiency of cordycepin is greatly improved due to the fact that the specific Cm1 and Cm2 genes are produced by utilizing the specific cordyceps militaris strain, and a green process for producing cord</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220819&DB=EPODOC&CC=CN&NR=114921354A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220819&DB=EPODOC&CC=CN&NR=114921354A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>TAN HUIPING</creatorcontrib><creatorcontrib>FENG BAOMIN</creatorcontrib><creatorcontrib>KAN TUO</creatorcontrib><creatorcontrib>JIANG RONGSHUAI</creatorcontrib><creatorcontrib>WEI DAPENG</creatorcontrib><creatorcontrib>LI CHENYANG</creatorcontrib><creatorcontrib>WANG LIANG</creatorcontrib><creatorcontrib>LI XIANG</creatorcontrib><creatorcontrib>LI QIAN</creatorcontrib><creatorcontrib>LI XIUPING</creatorcontrib><creatorcontrib>CHENG YANGHAO</creatorcontrib><title>Engineering strain for producing cordycepin and cordycepin derivative 3 '-deoxyinosine as well as preparation method and application of engineering strain</title><description>The invention belongs to the field of genetic engineering and process engineering, and discloses an engineering strain for producing cordycepin and a cordycepin derivative 3 '-deoxyinosine as well as a preparation method and application of the engineering strain. 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Comprising codon optimized genes Cm1 and Cm2, a gene amplification and recovery fragment, a methanol induced promoter fragment and a terminator fragment, an expression vector is connected to the genes and then transferred into pichia pastoris for screening, and the pichia pastoris engineering strain protected by the invention is obtained and can be applied to production of cordycepin and derivatives thereof. The engineering strain prepared by the preparation method has huge advantages in production of cordycepin and derivatives thereof, the production efficiency of cordycepin is greatly improved due to the fact that the specific Cm1 and Cm2 genes are produced by utilizing the specific cordyceps militaris strain, and a green process for producing cord</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Engineering strain for producing cordycepin and cordycepin derivative 3 '-deoxyinosine as well as preparation method and application of engineering strain |
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