Paper-based synthetic gene networks

Disclosed herein are shelf-stable compositions based on synthetic gene networks and/or cell-free systems lyophilized on a solid support. The composition can be easily transported and stored for a period of time and can be activated by simple addition of water. Methods of use, including but not limited to sensing and various logic functions, are also disclosed herein. The invention makes it possible to simply, aseptically and non-biologically configure synthetic biology-based techniques to clinical settings, food processing and industrial, military and consumer products. 本文公开的是在固体支持物上冻干的基于合成基因网络和/或无细胞体系的耐储存的组合物。所述组合物可以很容易地运输和储存一段时间，并可以通过简单地加入水而得以活化。本文还公开了使用方法，包括但不限于传感和各种逻辑功能。本发明使得能够将基于合成生物学的技术简单、无菌和非生物地配置至临床设置、食品加工和工业、军用和消费产品。