Matching compensation method and device for focused sequence image, and storage medium

The invention discloses a matching compensation method and device for focus sequence images, and a storage medium. The method comprises the following steps: selecting an original image of which the definition meets a preset requirement in original focus sequence images as a template image; an ROI area containing a target object is established in the template image, and image coordinates (xr, yr) of the ROI area are recorded; performing template matching on an original image by taking the template image as a search template, marking a region most similar to the ROI region in the original image as a corresponding search region, and recording image coordinates (xi, yi) of the search region; calculating a coordinate difference value (xi-xr, yi-yr) between the search region and the ROI region; compensating the original image according to the coordinate difference value (xi-xr, yi-yr) to obtain a compensated focusing sequence image; the problem of mismatching caused by non-vertical installation and non-vertical dis