Water-in-oil-in-water adjuvant for livestock animal vaccine as well as preparation method and application of water-in-oil-in-water adjuvant

The invention provides a water-in-oil-in-water adjuvant for a livestock animal vaccine as well as a preparation method and application of the water-in-oil-in-water adjuvant, and belongs to the technical field of biological product animal vaccines. The provided water-in-oil-in-water adjuvant is prepared from the following raw materials in percentage by mass: 75 to 85 weight percent of oil for injection, 1 to 5 weight percent of refined span-80, 10 to 24 weight percent of refined tween-80 and 1 to 3 weight percent of an immunostimulation compound. A vaccine prepared from the water-in-oil-in-water adjuvant provided by the invention is stable in quality and high in safety, and can induce an organism to generate a longer-lasting and more efficient immune reaction, so that the water-in-oil-in-water adjuvant can be used as a safe and effective adjuvant for livestock vaccines and the like. 本发明提供一种畜类动物疫苗用水包油包水佐剂及其制备方法和应用，属于生物制品类动物疫苗技术领域。提供的水包油包水佐剂的原料按质量百分比计包括：75wt％-85wt％的注射用油、1wt％-5wt％的精制司本-80和10wt％-24wt％的精制吐温-80，还可包括