Preparation method and application of electrochemical immunosensor for detecting aflatoxin B1

The invention discloses a preparation method and application of an electrochemical immunosensor for detecting aflatoxin B1. According to the preparation method provided by the invention, curcumin is wrapped in ZIF-8 to form CCM (ZIF)-8, and then PDA and anti-mouse IgG are modified on the surface of the CCM (ZIF)-8, so that an electrochemical nanoprobe is obtained. By adding curcumin, the electrochemical nanoprobe is specifically combined with an AFB1 antibody on the surface of a modified electrode to generate an electrochemical signal, quantitative detection of AFB1 is realized, and by embedding the electrochemical nanoprobe in ZIF-8, the water solubility and the stability of the electrochemical nanoprobe are improved, so that the stability of the electrochemical nanoprobe is good; pDA (Personal Digital Assistant) and anti-mouse IgG (Immunoglobulin G) are modified on the surface of the CCM (at) ZIF8 to serve as a signal amplification probe, so that the prepared electrochemical immunosensor is high in sensitiv