Apple drought tolerance negative regulation gene MdbHLH108 and application thereof

The invention discloses an apple drought tolerance negative regulation gene MdbHLH108 and application thereof. The nucleotide sequence of the apple gene MdbHLH108 is shown as SEQ ID NO. 1, and the amino acid sequence of the protein is shown as SEQ ID NO. 2. A DNA fragment of a complete coding segmen...

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Bibliographische Detailangaben
Hauptverfasser:	JIAO JIAN, BAI TUANHUI, ZHENG XIANBO, SONG CHUNHUI, WANG MEIGE, SONG SHANGWEI, SONG XUELIAN, WANG MIAOMIAO
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM ORGANIC CHEMISTRY PEPTIDES PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses an apple drought tolerance negative regulation gene MdbHLH108 and application thereof. The nucleotide sequence of the apple gene MdbHLH108 is shown as SEQ ID NO. 1, and the amino acid sequence of the protein is shown as SEQ ID NO. 2. A DNA fragment of a complete coding segment of the resistance-related gene MdbHLH108 is separated and cloned from a Malus hupehensis seedling through a plant gene engineering technology, the function of the gene is verified, and it is found that the drought resistance of a transgenic material is reduced and the ABA sensitivity is obviously improved after overexpression, which indicates that the gene MdbHLH108 plays a negative regulation role in drought, and the resistance-related gene MdbHLH108 has a good application prospect. The negative regulatory factor has the potential to be applied to gene editing and cultivation of drought-resistant new varieties, and has important significance on apple drought-resistant directional genetic improvement and stock br