Method for simultaneously determining six related steroid hormones of CAH

Method for simultaneously determining six related steroid hormones of CAH

The invention relates to the field of screening of congenital adrenal hyperplasia, in particular to a method for simultaneously determining six related steroid hormones of CAH. The six related steroid hormones are 17 alpha-hydroxyprogesterone, androstenedione, 11-deoxycortisol, 21-deoxycortisol, cor...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: TIAN PEIRUN, ZHAO MEI, CHEN FU, FANG BINGXIONG, ZHAO WEI, CAI MIANSHAN, LIU QI
Format: Patent
Sprache:chi ; eng
Schlagworte:
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page
container_title
container_volume
creator TIAN PEIRUN
ZHAO MEI
CHEN FU
FANG BINGXIONG
ZHAO WEI
CAI MIANSHAN
LIU QI
description The invention relates to the field of screening of congenital adrenal hyperplasia, in particular to a method for simultaneously determining six related steroid hormones of CAH. The six related steroid hormones are 17 alpha-hydroxyprogesterone, androstenedione, 11-deoxycortisol, 21-deoxycortisol, cortisol and corticosterone; the determination method comprises the following steps: S1, respectively preparing a mixed internal standard product, a quality control product and a standard curve product; s2, preparing a sample extracting solution; s3, extracting and incubating a sample; s4, concentrating the redissolved sample; and S5, determining the reconstitution fluid by using a liquid chromatography tandem mass spectrometer, drawing a calibration curve, and calculating the concentrations of 17 alpha-hydroxyprogesterone, androstenedione, 11-deoxycortisol, 21-deoxycortisol, cortisol and corticosterone in the sample to be detected. The CAH screening specificity and the positive predicted value can be effectively impr
format Patent
fullrecord <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN114689740A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN114689740A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN114689740A3</originalsourceid><addsrcrecordid>eNrjZPD0TS3JyE9RSMsvUijOzC3NKUnMS80vLc6pVEhJLUktys3My8xLB0pVKBSl5iSWpKYoFAOF8zNTFDLyi3Lz81KLFfLTFJwdPXgYWNMSc4pTeaE0N4Oim2uIs4duakF-fGpxQWJyal5qSbyzn6GhiZmFpbmJgaMxMWoAElw05w</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Method for simultaneously determining six related steroid hormones of CAH</title><source>esp@cenet</source><creator>TIAN PEIRUN ; ZHAO MEI ; CHEN FU ; FANG BINGXIONG ; ZHAO WEI ; CAI MIANSHAN ; LIU QI</creator><creatorcontrib>TIAN PEIRUN ; ZHAO MEI ; CHEN FU ; FANG BINGXIONG ; ZHAO WEI ; CAI MIANSHAN ; LIU QI</creatorcontrib><description>The invention relates to the field of screening of congenital adrenal hyperplasia, in particular to a method for simultaneously determining six related steroid hormones of CAH. The six related steroid hormones are 17 alpha-hydroxyprogesterone, androstenedione, 11-deoxycortisol, 21-deoxycortisol, cortisol and corticosterone; the determination method comprises the following steps: S1, respectively preparing a mixed internal standard product, a quality control product and a standard curve product; s2, preparing a sample extracting solution; s3, extracting and incubating a sample; s4, concentrating the redissolved sample; and S5, determining the reconstitution fluid by using a liquid chromatography tandem mass spectrometer, drawing a calibration curve, and calculating the concentrations of 17 alpha-hydroxyprogesterone, androstenedione, 11-deoxycortisol, 21-deoxycortisol, cortisol and corticosterone in the sample to be detected. The CAH screening specificity and the positive predicted value can be effectively impr</description><language>chi ; eng</language><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; PHYSICS ; TESTING</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20220701&amp;DB=EPODOC&amp;CC=CN&amp;NR=114689740A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,778,883,25547,76298</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20220701&amp;DB=EPODOC&amp;CC=CN&amp;NR=114689740A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>TIAN PEIRUN</creatorcontrib><creatorcontrib>ZHAO MEI</creatorcontrib><creatorcontrib>CHEN FU</creatorcontrib><creatorcontrib>FANG BINGXIONG</creatorcontrib><creatorcontrib>ZHAO WEI</creatorcontrib><creatorcontrib>CAI MIANSHAN</creatorcontrib><creatorcontrib>LIU QI</creatorcontrib><title>Method for simultaneously determining six related steroid hormones of CAH</title><description>The invention relates to the field of screening of congenital adrenal hyperplasia, in particular to a method for simultaneously determining six related steroid hormones of CAH. The six related steroid hormones are 17 alpha-hydroxyprogesterone, androstenedione, 11-deoxycortisol, 21-deoxycortisol, cortisol and corticosterone; the determination method comprises the following steps: S1, respectively preparing a mixed internal standard product, a quality control product and a standard curve product; s2, preparing a sample extracting solution; s3, extracting and incubating a sample; s4, concentrating the redissolved sample; and S5, determining the reconstitution fluid by using a liquid chromatography tandem mass spectrometer, drawing a calibration curve, and calculating the concentrations of 17 alpha-hydroxyprogesterone, androstenedione, 11-deoxycortisol, 21-deoxycortisol, cortisol and corticosterone in the sample to be detected. The CAH screening specificity and the positive predicted value can be effectively impr</description><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>PHYSICS</subject><subject>TESTING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZPD0TS3JyE9RSMsvUijOzC3NKUnMS80vLc6pVEhJLUktys3My8xLB0pVKBSl5iSWpKYoFAOF8zNTFDLyi3Lz81KLFfLTFJwdPXgYWNMSc4pTeaE0N4Oim2uIs4duakF-fGpxQWJyal5qSbyzn6GhiZmFpbmJgaMxMWoAElw05w</recordid><startdate>20220701</startdate><enddate>20220701</enddate><creator>TIAN PEIRUN</creator><creator>ZHAO MEI</creator><creator>CHEN FU</creator><creator>FANG BINGXIONG</creator><creator>ZHAO WEI</creator><creator>CAI MIANSHAN</creator><creator>LIU QI</creator><scope>EVB</scope></search><sort><creationdate>20220701</creationdate><title>Method for simultaneously determining six related steroid hormones of CAH</title><author>TIAN PEIRUN ; ZHAO MEI ; CHEN FU ; FANG BINGXIONG ; ZHAO WEI ; CAI MIANSHAN ; LIU QI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN114689740A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2022</creationdate><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>PHYSICS</topic><topic>TESTING</topic><toplevel>online_resources</toplevel><creatorcontrib>TIAN PEIRUN</creatorcontrib><creatorcontrib>ZHAO MEI</creatorcontrib><creatorcontrib>CHEN FU</creatorcontrib><creatorcontrib>FANG BINGXIONG</creatorcontrib><creatorcontrib>ZHAO WEI</creatorcontrib><creatorcontrib>CAI MIANSHAN</creatorcontrib><creatorcontrib>LIU QI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>TIAN PEIRUN</au><au>ZHAO MEI</au><au>CHEN FU</au><au>FANG BINGXIONG</au><au>ZHAO WEI</au><au>CAI MIANSHAN</au><au>LIU QI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for simultaneously determining six related steroid hormones of CAH</title><date>2022-07-01</date><risdate>2022</risdate><abstract>The invention relates to the field of screening of congenital adrenal hyperplasia, in particular to a method for simultaneously determining six related steroid hormones of CAH. The six related steroid hormones are 17 alpha-hydroxyprogesterone, androstenedione, 11-deoxycortisol, 21-deoxycortisol, cortisol and corticosterone; the determination method comprises the following steps: S1, respectively preparing a mixed internal standard product, a quality control product and a standard curve product; s2, preparing a sample extracting solution; s3, extracting and incubating a sample; s4, concentrating the redissolved sample; and S5, determining the reconstitution fluid by using a liquid chromatography tandem mass spectrometer, drawing a calibration curve, and calculating the concentrations of 17 alpha-hydroxyprogesterone, androstenedione, 11-deoxycortisol, 21-deoxycortisol, cortisol and corticosterone in the sample to be detected. The CAH screening specificity and the positive predicted value can be effectively impr</abstract><oa>free_for_read</oa></addata></record>
fulltext fulltext_linktorsrc
identifier
ispartof
issn
language chi ; eng
recordid cdi_epo_espacenet_CN114689740A
source esp@cenet
subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
title Method for simultaneously determining six related steroid hormones of CAH
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T03%3A01%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=TIAN%20PEIRUN&rft.date=2022-07-01&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN114689740A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true