Primer combination for PCR (Polymerase Chain Reaction) identification of bungarus multicinctus medicinal material, standard decoction and traditional Chinese medicine formula granules as well as application and identification method of primer combination

The invention discloses a primer combination for PCR (Polymerase Chain Reaction) identification of bungarus multicinctus medicinal materials, standard decoction and traditional Chinese medicine formula granules, the primer combination comprises a primer I and a primer II, the primer I is a DNA (Deoxyribose Nucleic Acid) molecule with a nucleotide sequence as shown in SEQ ID NO: 1, and the primer II is a DNA molecule with a nucleotide sequence as shown in SEQ ID NO: 2. The invention further discloses application of the primer combination and an identification method based on the primer combination. By implementing the method, the bungarus multicinctus medicinal material, the standard decoction and the traditional Chinese medicine formula granules can be effectively identified. 本发明公开了一种用于金钱白花蛇药材、标准汤剂及中药配方颗粒PCR鉴别的引物组合，其包括引物一和引物二，其中，引物一为核苷酸序列如SEQ ID NO：1所示的DNA分子，引物二为核苷酸序列如SEQ ID NO：2所示的DNA分子。本发明还公开了上述引物组合的应用，以及基于该引物组合的鉴别方法。实施本发明，可有效鉴别金钱白花蛇药材、标准汤剂以及中药配方颗粒。