Regeneration method of cyclosporine A chromatographic silica gel
The invention discloses a regeneration method of cyclosporine A chromatographic silica gel. The regeneration method comprises the following steps: (1) draining a residual solvent in a silica gel column after cyclosporine A is separated and purified; washing from the bottom of the silica gel column b...
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| creator | YANXING XU JIN CHAI XIAOTAO SHI HAO LI XIANGQIAN ZHANG JINFENG HUANG YE SHEN WANZHAO GUO SHAODING |
| description | The invention discloses a regeneration method of cyclosporine A chromatographic silica gel. The regeneration method comprises the following steps: (1) draining a residual solvent in a silica gel column after cyclosporine A is separated and purified; washing from the bottom of the silica gel column by using a high-grade solvent containing a nonionic surfactant; (2) firstly using deionized water containing hydrogen peroxide, and then using deionized water for flushing; and (3) draining water, taking out the silica gel, drying, and repacking. Compared with original silica gel, the regenerated silica gel has no difference in physical property, and after the regenerated silica gel is repeatedly used for many times, the yield of qualified cyclosporine A can reach 60%-70%.
本发明公开了一种环孢素A层析硅胶的再生方法，包括以下步骤：(1)将经分离提纯环孢素A后硅胶柱内残留的溶剂排干；使用含非离子表面活性剂的高级性溶剂从硅胶柱底部进行冲洗；(2)然后先使用含双氧水的去离子水，再使用去离子水进行冲洗；(3)排干水分，取出硅胶，烘干后重新装柱。本发明再生硅胶不仅在物理性质上与原硅胶相比没有差异，而且再生硅胶重复使用多次后，合格环孢素A的收率可以达到60％-70％。 |
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本发明公开了一种环孢素A层析硅胶的再生方法，包括以下步骤：(1)将经分离提纯环孢素A后硅胶柱内残留的溶剂排干；使用含非离子表面活性剂的高级性溶剂从硅胶柱底部进行冲洗；(2)然后先使用含双氧水的去离子水，再使用去离子水进行冲洗；(3)排干水分，取出硅胶，烘干后重新装柱。本发明再生硅胶不仅在物理性质上与原硅胶相比没有差异，而且再生硅胶重复使用多次后，合格环孢素A的收率可以达到60％-70％。</description><language>chi ; eng</language><subject>CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY ; PERFORMING OPERATIONS ; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL ; THEIR RELEVANT APPARATUS ; TRANSPORTING</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220624&DB=EPODOC&CC=CN&NR=114653350A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220624&DB=EPODOC&CC=CN&NR=114653350A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>YANXING</creatorcontrib><creatorcontrib>XU JIN</creatorcontrib><creatorcontrib>CHAI XIAOTAO</creatorcontrib><creatorcontrib>SHI HAO</creatorcontrib><creatorcontrib>LI XIANGQIAN</creatorcontrib><creatorcontrib>ZHANG JINFENG</creatorcontrib><creatorcontrib>HUANG YE</creatorcontrib><creatorcontrib>SHEN WANZHAO</creatorcontrib><creatorcontrib>GUO SHAODING</creatorcontrib><title>Regeneration method of cyclosporine A chromatographic silica gel</title><description>The invention discloses a regeneration method of cyclosporine A chromatographic silica gel. The regeneration method comprises the following steps: (1) draining a residual solvent in a silica gel column after cyclosporine A is separated and purified; washing from the bottom of the silica gel column by using a high-grade solvent containing a nonionic surfactant; (2) firstly using deionized water containing hydrogen peroxide, and then using deionized water for flushing; and (3) draining water, taking out the silica gel, drying, and repacking. Compared with original silica gel, the regenerated silica gel has no difference in physical property, and after the regenerated silica gel is repeatedly used for many times, the yield of qualified cyclosporine A can reach 60%-70%.
本发明公开了一种环孢素A层析硅胶的再生方法，包括以下步骤：(1)将经分离提纯环孢素A后硅胶柱内残留的溶剂排干；使用含非离子表面活性剂的高级性溶剂从硅胶柱底部进行冲洗；(2)然后先使用含双氧水的去离子水，再使用去离子水进行冲洗；(3)排干水分，取出硅胶，烘干后重新装柱。本发明再生硅胶不仅在物理性质上与原硅胶相比没有差异，而且再生硅胶重复使用多次后，合格环孢素A的收率可以达到60％-70％。</description><subject>CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY</subject><subject>PERFORMING OPERATIONS</subject><subject>PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL</subject><subject>THEIR RELEVANT APPARATUS</subject><subject>TRANSPORTING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZHAISk1PzUstSizJzM9TyE0tychPUchPU0iuTM7JLy7IL8rMS1VwVEjOKMrPTSzJTy9KLMjITFYozszJTE5USE_N4WFgTUvMKU7lhdLcDIpuriHOHrqpBfnxqcUFiclA40vinf0MDU3MTI2NTQ0cjYlRAwDrxzF7</recordid><startdate>20220624</startdate><enddate>20220624</enddate><creator>YANXING</creator><creator>XU JIN</creator><creator>CHAI XIAOTAO</creator><creator>SHI HAO</creator><creator>LI XIANGQIAN</creator><creator>ZHANG JINFENG</creator><creator>HUANG YE</creator><creator>SHEN WANZHAO</creator><creator>GUO SHAODING</creator><scope>EVB</scope></search><sort><creationdate>20220624</creationdate><title>Regeneration method of cyclosporine A chromatographic silica gel</title><author>YANXING ; XU JIN ; CHAI XIAOTAO ; SHI HAO ; LI XIANGQIAN ; ZHANG JINFENG ; HUANG YE ; SHEN WANZHAO ; GUO SHAODING</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN114653350A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2022</creationdate><topic>CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY</topic><topic>PERFORMING OPERATIONS</topic><topic>PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL</topic><topic>THEIR RELEVANT APPARATUS</topic><topic>TRANSPORTING</topic><toplevel>online_resources</toplevel><creatorcontrib>YANXING</creatorcontrib><creatorcontrib>XU JIN</creatorcontrib><creatorcontrib>CHAI XIAOTAO</creatorcontrib><creatorcontrib>SHI HAO</creatorcontrib><creatorcontrib>LI XIANGQIAN</creatorcontrib><creatorcontrib>ZHANG JINFENG</creatorcontrib><creatorcontrib>HUANG YE</creatorcontrib><creatorcontrib>SHEN WANZHAO</creatorcontrib><creatorcontrib>GUO SHAODING</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>YANXING</au><au>XU JIN</au><au>CHAI XIAOTAO</au><au>SHI HAO</au><au>LI XIANGQIAN</au><au>ZHANG JINFENG</au><au>HUANG YE</au><au>SHEN WANZHAO</au><au>GUO SHAODING</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Regeneration method of cyclosporine A chromatographic silica gel</title><date>2022-06-24</date><risdate>2022</risdate><abstract>The invention discloses a regeneration method of cyclosporine A chromatographic silica gel. The regeneration method comprises the following steps: (1) draining a residual solvent in a silica gel column after cyclosporine A is separated and purified; washing from the bottom of the silica gel column by using a high-grade solvent containing a nonionic surfactant; (2) firstly using deionized water containing hydrogen peroxide, and then using deionized water for flushing; and (3) draining water, taking out the silica gel, drying, and repacking. Compared with original silica gel, the regenerated silica gel has no difference in physical property, and after the regenerated silica gel is repeatedly used for many times, the yield of qualified cyclosporine A can reach 60%-70%.
本发明公开了一种环孢素A层析硅胶的再生方法，包括以下步骤：(1)将经分离提纯环孢素A后硅胶柱内残留的溶剂排干；使用含非离子表面活性剂的高级性溶剂从硅胶柱底部进行冲洗；(2)然后先使用含双氧水的去离子水，再使用去离子水进行冲洗；(3)排干水分，取出硅胶，烘干后重新装柱。本发明再生硅胶不仅在物理性质上与原硅胶相比没有差异，而且再生硅胶重复使用多次后，合格环孢素A的收率可以达到60％-70％。</abstract><oa>free_for_read</oa></addata></record> |
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| title | Regeneration method of cyclosporine A chromatographic silica gel |
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