Near-infrared fluorescent probe taking benzylboronic acid pinacol ester as detection group as well as preparation method and application of near-infrared fluorescent probe

The invention provides a near-infrared fluorescent probe taking benzylboronic acid pinacol ester as a detection group as well as a preparation method and application of the near-infrared fluorescent probe, aiming at overcoming the defects that most of existing nitrosyl peroxide anion detection probes are low in sensitivity, high in detection limit, relatively poor in water solubility and relatively short in fluorescence emission wavelength. The near-infrared fluorescent probe designed by the invention is relatively low in light scattering level, autofluorescence of biological cells can be reduced, larger tissue penetration can be realized, and rapid, sensitive, efficient and specific detection of the nitrosyl peroxide anions can be realized through a fluorescence emission spectrum. 本发明提供一种以苄基硼酸频哪醇酯类为检测基团的近红外荧光探针及其制备方法和应用，解决现有过氧化亚硝酰阴离子检测探针大多存在灵敏度低、检出极限高、水溶性较差、荧光发射波长较短的不足之处。本发明设计的近红外荧光探针光散射水平较低，可以降低生物细胞的自发荧光，实现更大的组织穿透，可以通过荧光发射光谱实现对过氧化亚硝酰阴离子的快速、灵敏、高效、专一性检测。