CD7-based humanized chimeric antigen receptor and application thereof

The invention relates to a humanized chimeric antigen receptor based on CD7 and application thereof, in particular to a construction method of a chimeric antigen receptor T (CAR-T) cell technology based on a tumor specific target spot CD7 and application of the chimeric antigen receptor T in anti-tumor treatment. The chimeric antigen receptor is formed by connecting an antigen binding structural domain, a transmembrane structural domain, a costimulatory signal transduction region and a CD3 zeta signal transduction structural domain in series, wherein the antigen binding structural domain is bound with a tumor surface antigen, and the tumor surface antigen is CD7. According to the humanized chimeric antigen receptor disclosed by the invention, specific gene modification is carried out on the single-chain antibody with specificity to the CD7 antigen, the modified humanized single-chain antibody can enable the antigen-antibody binding force to be stronger, the stimulation of the chimeric receptor to T cells is m