Method for enhancing regulatory protein expression to improve fermentation level of glutamine transaminase
The invention discloses a method for enhancing regulatory protein expression to improve the fermentation level of glutamine transaminase, which comprises the following steps: in streptomyces mobaraensis C2, over-expressing regulatory protein genes SMDS4036, SMDS2341 and SMDS3961 respectively by usin...
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| creator | BAI LINQUAN BU JIANGUO LIU XIAN |
| description | The invention discloses a method for enhancing regulatory protein expression to improve the fermentation level of glutamine transaminase, which comprises the following steps: in streptomyces mobaraensis C2, over-expressing regulatory protein genes SMDS4036, SMDS2341 and SMDS3961 respectively by using a strong promoter kasOp * to obtain a mutant strain with improved TG enzyme yield. The positive regulation can be enhanced by enhancing the expression of the regulation protein gene, so that the yield of the TG enzyme is improved. The TG enzyme fermentation final yields of the engineering strains LX-55, LX-56 and LX-58 obtained in the embodiment of the invention are respectively improved by 33.33%, 16.65% and 69.76% compared with those of the wild strains. According to the method, the fermentation yield of the TG enzyme can be remarkably increased, and meanwhile, the fermentation cost is greatly reduced.
本发明公开了一种增强调控蛋白表达以提高谷氨酰胺转氨酶发酵水平的方法，是通过在茂原链霉菌C2中，利用强启动子kasOp*分别过量表达调控蛋白基因SMDS_4036、SMDS_2341、SMDS_3961，得到TG酶产量提高 |
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本发明公开了一种增强调控蛋白表达以提高谷氨酰胺转氨酶发酵水平的方法，是通过在茂原链霉菌C2中，利用强启动子kasOp*分别过量表达调控蛋白基因SMDS_4036、SMDS_2341、SMDS_3961，得到TG酶产量提高</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220527&DB=EPODOC&CC=CN&NR=114540397A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,777,882,25545,76296</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220527&DB=EPODOC&CC=CN&NR=114540397A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>BAI LINQUAN</creatorcontrib><creatorcontrib>BU JIANGUO</creatorcontrib><creatorcontrib>LIU XIAN</creatorcontrib><title>Method for enhancing regulatory protein expression to improve fermentation level of glutamine transaminase</title><description>The invention discloses a method for enhancing regulatory protein expression to improve the fermentation level of glutamine transaminase, which comprises the following steps: in streptomyces mobaraensis C2, over-expressing regulatory protein genes SMDS4036, SMDS2341 and SMDS3961 respectively by using a strong promoter kasOp * to obtain a mutant strain with improved TG enzyme yield. The positive regulation can be enhanced by enhancing the expression of the regulation protein gene, so that the yield of the TG enzyme is improved. The TG enzyme fermentation final yields of the engineering strains LX-55, LX-56 and LX-58 obtained in the embodiment of the invention are respectively improved by 33.33%, 16.65% and 69.76% compared with those of the wild strains. According to the method, the fermentation yield of the TG enzyme can be remarkably increased, and meanwhile, the fermentation cost is greatly reduced.
本发明公开了一种增强调控蛋白表达以提高谷氨酰胺转氨酶发酵水平的方法，是通过在茂原链霉菌C2中，利用强启动子kasOp*分别过量表达调控蛋白基因SMDS_4036、SMDS_2341、SMDS_3961，得到TG酶产量提高</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNirEKwkAQBdNYiPoP6wcIhkTEUoJio5V9WOK75OSyd9xtgv69BvwAqxmGmWfPK7TzDzI-EqRjaay0FNEOjtXHN4XoFVYIrxCRkvVC6sn23z6CDGIPUdapO4xw5A21blDurYA0sqRJOWGZzQy7hNWPi2x9Pt2rywbB10iBGwi0rm55Xu7KbXHYH4t_ng9IMkJx</recordid><startdate>20220527</startdate><enddate>20220527</enddate><creator>BAI LINQUAN</creator><creator>BU JIANGUO</creator><creator>LIU XIAN</creator><scope>EVB</scope></search><sort><creationdate>20220527</creationdate><title>Method for enhancing regulatory protein expression to improve fermentation level of glutamine transaminase</title><author>BAI LINQUAN ; BU JIANGUO ; LIU XIAN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN114540397A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2022</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>BAI LINQUAN</creatorcontrib><creatorcontrib>BU JIANGUO</creatorcontrib><creatorcontrib>LIU XIAN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>BAI LINQUAN</au><au>BU JIANGUO</au><au>LIU XIAN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for enhancing regulatory protein expression to improve fermentation level of glutamine transaminase</title><date>2022-05-27</date><risdate>2022</risdate><abstract>The invention discloses a method for enhancing regulatory protein expression to improve the fermentation level of glutamine transaminase, which comprises the following steps: in streptomyces mobaraensis C2, over-expressing regulatory protein genes SMDS4036, SMDS2341 and SMDS3961 respectively by using a strong promoter kasOp * to obtain a mutant strain with improved TG enzyme yield. The positive regulation can be enhanced by enhancing the expression of the regulation protein gene, so that the yield of the TG enzyme is improved. The TG enzyme fermentation final yields of the engineering strains LX-55, LX-56 and LX-58 obtained in the embodiment of the invention are respectively improved by 33.33%, 16.65% and 69.76% compared with those of the wild strains. According to the method, the fermentation yield of the TG enzyme can be remarkably increased, and meanwhile, the fermentation cost is greatly reduced.
本发明公开了一种增强调控蛋白表达以提高谷氨酰胺转氨酶发酵水平的方法，是通过在茂原链霉菌C2中，利用强启动子kasOp*分别过量表达调控蛋白基因SMDS_4036、SMDS_2341、SMDS_3961，得到TG酶产量提高</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for enhancing regulatory protein expression to improve fermentation level of glutamine transaminase |
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