Tumor cell PD-L1PD-L2 immunofluorescence + FISH (fluorescence in situ hybridization) detection kit

The invention relates to the technical field of medical biology, in particular to a tumor cell PD-L1PD-L2 immunofluorescence + FISH (fluorescence in situ hybridization) detection kit, which comprises Buffer-I, Buffer-II, Buffer-III, confining liquid, a PD-1/PD-L1 antibody, a CD45 antibody, a second antibody, antibody diluent, DAPI (4, 4 '-diaminobenzene), a positive pair photograph, 10 * CF2 stationary liquid, a probe, formamide working liquid, 20 * SSC (solid state complex) and a BSA (bovine serum albumin) solution. The use method comprises the following steps: S1, sampling blood and other body fluids: for blood sampling, adopting a 5mL vacuum blood collection tube with EDTA, sodium citrate, potassium oxalate/sodium fluoride, heparin and ACD anticoagulation, and immediately reversing and uniformly mixing for 8 times after blood collection is finished; for other body fluid, hydrothorax and ascites, cerebrospinal fluid and lavage fluid which are extracted clinically are injected into the bottle containing the