Molecular marker, primer and method for identifying hippocampus kelloggi genetic sex and application
The invention discloses a molecular marker for identifying genetic sex of hippocampus kelloggi and a method for identifying male and female hippocampus kelloggi. The sequence of the molecular marker is SEQ ID NO. 1. The hippocampus kelloggi genetic sex identification method is simple, convenient and...
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| creator | JIA CHENGXI YUAN LILAI DONG SHAOJIE YANG YANJING SUN JINHUI MA JINGJING CUI PEI |
| description | The invention discloses a molecular marker for identifying genetic sex of hippocampus kelloggi and a method for identifying male and female hippocampus kelloggi. The sequence of the molecular marker is SEQ ID NO. 1. The hippocampus kelloggi genetic sex identification method is simple, convenient and rapid to operate, samples are collected in a mode of cutting a small number of dorsal fins, and damage to the hippocampus kelloggi is small; the genome is prepared by adopting an alkali lysis method, so that the yield of DNA is high, and the requirement on a tissue sample is low; the sex identification molecular marker and the identification method provided by the invention are high in identification result accuracy, are not influenced by tissue specificity and environment, and have important value for sex control and all-male fry breeding of the hippocampus kelloggi.
本发明公开了一种鉴别线纹海马遗传性别的分子标记及雌雄线纹海马鉴定方法，所述分子标记的序列为SEQ ID NO.1。本发明线纹海马遗传性别鉴定方法操作简便快捷，样品收集采用剪少量背鳍的方式，对线纹海马损伤小；基因组制备采用碱裂解法，DNA收获量高，对组织样品的要求低；本发明提供的性别鉴定分子标记和 |
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本发明公开了一种鉴别线纹海马遗传性别的分子标记及雌雄线纹海马鉴定方法，所述分子标记的序列为SEQ ID NO.1。本发明线纹海马遗传性别鉴定方法操作简便快捷，样品收集采用剪少量背鳍的方式，对线纹海马损伤小；基因组制备采用碱裂解法，DNA收获量高，对组织样品的要求低；本发明提供的性别鉴定分子标记和</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220513&DB=EPODOC&CC=CN&NR=114480601A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220513&DB=EPODOC&CC=CN&NR=114480601A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>JIA CHENGXI</creatorcontrib><creatorcontrib>YUAN LILAI</creatorcontrib><creatorcontrib>DONG SHAOJIE</creatorcontrib><creatorcontrib>YANG YANJING</creatorcontrib><creatorcontrib>SUN JINHUI</creatorcontrib><creatorcontrib>MA JINGJING</creatorcontrib><creatorcontrib>CUI PEI</creatorcontrib><title>Molecular marker, primer and method for identifying hippocampus kelloggi genetic sex and application</title><description>The invention discloses a molecular marker for identifying genetic sex of hippocampus kelloggi and a method for identifying male and female hippocampus kelloggi. The sequence of the molecular marker is SEQ ID NO. 1. The hippocampus kelloggi genetic sex identification method is simple, convenient and rapid to operate, samples are collected in a mode of cutting a small number of dorsal fins, and damage to the hippocampus kelloggi is small; the genome is prepared by adopting an alkali lysis method, so that the yield of DNA is high, and the requirement on a tissue sample is low; the sex identification molecular marker and the identification method provided by the invention are high in identification result accuracy, are not influenced by tissue specificity and environment, and have important value for sex control and all-male fry breeding of the hippocampus kelloggi.
本发明公开了一种鉴别线纹海马遗传性别的分子标记及雌雄线纹海马鉴定方法，所述分子标记的序列为SEQ ID NO.1。本发明线纹海马遗传性别鉴定方法操作简便快捷，样品收集采用剪少量背鳍的方式，对线纹海马损伤小；基因组制备采用碱裂解法，DNA收获量高，对组织样品的要求低；本发明提供的性别鉴定分子标记和</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNy7EKwjAQgOEuDqK-w7krWCziKkVx0cm9HMk1PZrkjiQFfXtRfACnf_m_eWVv4slMHhMETCOlDWjiQAkwWghUBrHQSwK2FAv3L44OBlYVg0GnDCN5L84xOIpU2ECm59eiqmeDhSUuq1mPPtPq10W1vpwf7XVLKh1lRfOxXXuv66Y57g67-rT_53kDBp8_IA</recordid><startdate>20220513</startdate><enddate>20220513</enddate><creator>JIA CHENGXI</creator><creator>YUAN LILAI</creator><creator>DONG SHAOJIE</creator><creator>YANG YANJING</creator><creator>SUN JINHUI</creator><creator>MA JINGJING</creator><creator>CUI PEI</creator><scope>EVB</scope></search><sort><creationdate>20220513</creationdate><title>Molecular marker, primer and method for identifying hippocampus kelloggi genetic sex and application</title><author>JIA CHENGXI ; YUAN LILAI ; DONG SHAOJIE ; YANG YANJING ; SUN JINHUI ; MA JINGJING ; CUI PEI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN114480601A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2022</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>JIA CHENGXI</creatorcontrib><creatorcontrib>YUAN LILAI</creatorcontrib><creatorcontrib>DONG SHAOJIE</creatorcontrib><creatorcontrib>YANG YANJING</creatorcontrib><creatorcontrib>SUN JINHUI</creatorcontrib><creatorcontrib>MA JINGJING</creatorcontrib><creatorcontrib>CUI PEI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>JIA CHENGXI</au><au>YUAN LILAI</au><au>DONG SHAOJIE</au><au>YANG YANJING</au><au>SUN JINHUI</au><au>MA JINGJING</au><au>CUI PEI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Molecular marker, primer and method for identifying hippocampus kelloggi genetic sex and application</title><date>2022-05-13</date><risdate>2022</risdate><abstract>The invention discloses a molecular marker for identifying genetic sex of hippocampus kelloggi and a method for identifying male and female hippocampus kelloggi. The sequence of the molecular marker is SEQ ID NO. 1. The hippocampus kelloggi genetic sex identification method is simple, convenient and rapid to operate, samples are collected in a mode of cutting a small number of dorsal fins, and damage to the hippocampus kelloggi is small; the genome is prepared by adopting an alkali lysis method, so that the yield of DNA is high, and the requirement on a tissue sample is low; the sex identification molecular marker and the identification method provided by the invention are high in identification result accuracy, are not influenced by tissue specificity and environment, and have important value for sex control and all-male fry breeding of the hippocampus kelloggi.
本发明公开了一种鉴别线纹海马遗传性别的分子标记及雌雄线纹海马鉴定方法，所述分子标记的序列为SEQ ID NO.1。本发明线纹海马遗传性别鉴定方法操作简便快捷，样品收集采用剪少量背鳍的方式，对线纹海马损伤小；基因组制备采用碱裂解法，DNA收获量高，对组织样品的要求低；本发明提供的性别鉴定分子标记和</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Molecular marker, primer and method for identifying hippocampus kelloggi genetic sex and application |
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