SgRNA for specifically recognizing pig Wip1 gene and application and product thereof
The invention provides sgRNA for specific recognition of a pig Wip1 gene and application and a product thereof, and relates to the technical field of genetic engineering, the sgRNA for specific recognition of the pig Wip1 gene provided by the invention can specifically recognize the front 1500bp of...
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| creator | FENG ZHENG LIU ZHIGUO WANG YUE LI KUI MOU YULIAN |
| description | The invention provides sgRNA for specific recognition of a pig Wip1 gene and application and a product thereof, and relates to the technical field of genetic engineering, the sgRNA for specific recognition of the pig Wip1 gene provided by the invention can specifically recognize the front 1500bp of a promoter of the pig Wip1 gene through selection of a specific sequence, and the sgRNA provided by the invention is used for a CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9) system. It is found through experiments that the sgRNA is matched with a CRISPR/Cas9 system, the knockout efficiency is 35%, 40% and 43%, gene editing operation can be efficiently conducted on the pig Wip1 gene, and the application in the aspects of gene function research, disease resistance breeding and the like of pig cells or individuals can be achieved.
本发明提供了一种特异性识别猪Wip1基因的sgRNA及其应用和产品，涉及基因工程技术领域，本发明提供的特异性识别猪Wip1基因的sgRNA通过特定序列的选择，能够特异性识别猪Wip1基因的启动子前1500bp，且本发明提供的sgRNA用于CRISPR/Cas9系统。本发明 |
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本发明提供了一种特异性识别猪Wip1基因的sgRNA及其应用和产品，涉及基因工程技术领域，本发明提供的特异性识别猪Wip1基因的sgRNA通过特定序列的选择，能够特异性识别猪Wip1基因的启动子前1500bp，且本发明提供的sgRNA用于CRISPR/Cas9系统。本发明</description><language>chi ; eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; BEER ; BIOCHEMISTRY ; CARE OF BIRDS, FISHES, INSECTS ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; NEW BREEDS OF ANIMALS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR ; SPIRITS ; TRAPPING ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220513&DB=EPODOC&CC=CN&NR=114480397A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220513&DB=EPODOC&CC=CN&NR=114480397A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>FENG ZHENG</creatorcontrib><creatorcontrib>LIU ZHIGUO</creatorcontrib><creatorcontrib>WANG YUE</creatorcontrib><creatorcontrib>LI KUI</creatorcontrib><creatorcontrib>MOU YULIAN</creatorcontrib><title>SgRNA for specifically recognizing pig Wip1 gene and application and product thereof</title><description>The invention provides sgRNA for specific recognition of a pig Wip1 gene and application and a product thereof, and relates to the technical field of genetic engineering, the sgRNA for specific recognition of the pig Wip1 gene provided by the invention can specifically recognize the front 1500bp of a promoter of the pig Wip1 gene through selection of a specific sequence, and the sgRNA provided by the invention is used for a CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9) system. It is found through experiments that the sgRNA is matched with a CRISPR/Cas9 system, the knockout efficiency is 35%, 40% and 43%, gene editing operation can be efficiently conducted on the pig Wip1 gene, and the application in the aspects of gene function research, disease resistance breeding and the like of pig cells or individuals can be achieved.
本发明提供了一种特异性识别猪Wip1基因的sgRNA及其应用和产品，涉及基因工程技术领域，本发明提供的特异性识别猪Wip1基因的sgRNA通过特定序列的选择，能够特异性识别猪Wip1基因的启动子前1500bp，且本发明提供的sgRNA用于CRISPR/Cas9系统。本发明</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CARE OF BIRDS, FISHES, INSECTS</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>NEW BREEDS OF ANIMALS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR</subject><subject>SPIRITS</subject><subject>TRAPPING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjEEKwjAQAHPxIOof1gcIhha0x1IUTz1owWMJ6SYuhOySxIO-3iI-wNMwMMxSDTd_7VtwnCALWnJkTQgvSGjZR3pT9CDk4U6iwWNEMHECIxLmsBDHr0vi6WkLlAcmZLdWC2dCxs2PK7U9n4buskPhEbMYO4_K2PVa1_VxXzWHtvqn-QA_tjhG</recordid><startdate>20220513</startdate><enddate>20220513</enddate><creator>FENG ZHENG</creator><creator>LIU ZHIGUO</creator><creator>WANG YUE</creator><creator>LI KUI</creator><creator>MOU YULIAN</creator><scope>EVB</scope></search><sort><creationdate>20220513</creationdate><title>SgRNA for specifically recognizing pig Wip1 gene and application and product thereof</title><author>FENG ZHENG ; LIU ZHIGUO ; WANG YUE ; LI KUI ; MOU YULIAN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN114480397A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2022</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CARE OF BIRDS, FISHES, INSECTS</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>NEW BREEDS OF ANIMALS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR</topic><topic>SPIRITS</topic><topic>TRAPPING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>FENG ZHENG</creatorcontrib><creatorcontrib>LIU ZHIGUO</creatorcontrib><creatorcontrib>WANG YUE</creatorcontrib><creatorcontrib>LI KUI</creatorcontrib><creatorcontrib>MOU YULIAN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>FENG ZHENG</au><au>LIU ZHIGUO</au><au>WANG YUE</au><au>LI KUI</au><au>MOU YULIAN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>SgRNA for specifically recognizing pig Wip1 gene and application and product thereof</title><date>2022-05-13</date><risdate>2022</risdate><abstract>The invention provides sgRNA for specific recognition of a pig Wip1 gene and application and a product thereof, and relates to the technical field of genetic engineering, the sgRNA for specific recognition of the pig Wip1 gene provided by the invention can specifically recognize the front 1500bp of a promoter of the pig Wip1 gene through selection of a specific sequence, and the sgRNA provided by the invention is used for a CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9) system. It is found through experiments that the sgRNA is matched with a CRISPR/Cas9 system, the knockout efficiency is 35%, 40% and 43%, gene editing operation can be efficiently conducted on the pig Wip1 gene, and the application in the aspects of gene function research, disease resistance breeding and the like of pig cells or individuals can be achieved.
本发明提供了一种特异性识别猪Wip1基因的sgRNA及其应用和产品，涉及基因工程技术领域，本发明提供的特异性识别猪Wip1基因的sgRNA通过特定序列的选择，能够特异性识别猪Wip1基因的启动子前1500bp，且本发明提供的sgRNA用于CRISPR/Cas9系统。本发明</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CARE OF BIRDS, FISHES, INSECTS CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW BREEDS OF ANIMALS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR SPIRITS TRAPPING VINEGAR WINE |
| title | SgRNA for specifically recognizing pig Wip1 gene and application and product thereof |
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