Method for purifying four polysaccharide products from heparin sodium by-products

Method for purifying four polysaccharide products from heparin sodium by-products

The invention discloses a method for purifying four polysaccharide products from a heparin sodium by-product. The four polysaccharide products including chondroitin sulfate, dermatan sulfate, heparinoid and sulodexide are respectively obtained by the steps of fractional precipitation, hydrogen perox...

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Hauptverfasser: DU XI, CUI JIE, HU LINA, QIE ZHENGGANG, LI ZHIMIN, BAI WENJU, LI XIAOPENG, TIAN ZHIPENG
Format: Patent
Sprache:chi ; eng
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CHEMISTRY
COMPOSITIONS BASED THEREON
DERIVATIVES THEREOF
METALLURGY
ORGANIC MACROMOLECULAR COMPOUNDS
POLYSACCHARIDES
THEIR PREPARATION OR CHEMICAL WORKING-UP
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creator DU XI
CUI JIE
HU LINA
QIE ZHENGGANG
LI ZHIMIN
BAI WENJU
LI XIAOPENG
TIAN ZHIPENG
description The invention discloses a method for purifying four polysaccharide products from a heparin sodium by-product. The four polysaccharide products including chondroitin sulfate, dermatan sulfate, heparinoid and sulodexide are respectively obtained by the steps of fractional precipitation, hydrogen peroxide oxidation, ultrafiltration, freeze-drying and the like on the heparin sodium by-product. According to the method, the optimal condition of ethanol precipitation at room temperature is determined, energy consumption caused by low-temperature precipitation and high-temperature precipitation is avoided, and the yield of four polysaccharide products is increased by about 10%; by further optimizing the oxidation process, pigments, proteins and nucleic acid impurities are effectively removed by oxidizing for 3-10 hours at the temperature of 35-40 DEG C, the time is saved by 50% compared with the current oxidation technology of oxidizing for 6-24 hours by hydrogen peroxide at the temperature of 30 DEG C, and the produ
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The four polysaccharide products including chondroitin sulfate, dermatan sulfate, heparinoid and sulodexide are respectively obtained by the steps of fractional precipitation, hydrogen peroxide oxidation, ultrafiltration, freeze-drying and the like on the heparin sodium by-product. 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subjects CHEMISTRY
COMPOSITIONS BASED THEREON
DERIVATIVES THEREOF
METALLURGY
ORGANIC MACROMOLECULAR COMPOUNDS
POLYSACCHARIDES
THEIR PREPARATION OR CHEMICAL WORKING-UP
title Method for purifying four polysaccharide products from heparin sodium by-products
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