Method for identifying Bupleurum marginatum and/or Bupleurum harmala based on SCAR molecular marker

The invention discloses a method for identifying Bupleurum marginatum and/or Bupleurum harmala based on an SCAR (sequence characterized amplified region) molecular marker. The nucleotide sequence of the SCAR molecular marker is as shown in SEQ ID No.1; the sequence of a forward primer of the primer...

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Bibliographische Detailangaben
Hauptverfasser: HUANG RONG, XIE XUENA, CHAO ZHI, LIU HONG, ZHENG RUWEN, DU HAIYONG, JIANG YANFENGYANG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a method for identifying Bupleurum marginatum and/or Bupleurum harmala based on an SCAR (sequence characterized amplified region) molecular marker. The nucleotide sequence of the SCAR molecular marker is as shown in SEQ ID No.1; the sequence of a forward primer of the primer pair is as shown in SEQ ID No.2, and the sequence of a reverse primer of the primer pair is as shown in SEQ ID No.3. The SCAR molecular marker and the primer pair disclosed by the invention can specifically and accurately identify the bupleurum marginatum and/or the bupleurum tenuifolium, provide a research basis for development, utilization and protection of the bupleurum marginatum and/or the bupleurum tenuifolium, and also provide reference for hereditary characteristics and identification of other traditional Chinese medicinal materials. 本发明公开了一种基于SCAR分子标记鉴定竹叶柴胡和/或窄竹叶柴胡的方法,所述SCAR分子标记的核苷酸序列如SEQ ID No.1所示;所述引物对的正向引物的序列如SEQ ID No.2所示,反向引物的序列如SEQ ID No.3所示。本发明的SCAR分子标记、以及引物对,可以特异性地、准确地鉴定竹叶柴胡和/或窄竹叶柴胡,为竹叶柴胡和/或窄竹叶柴胡的开