Method for pre-amplifying multi-target nucleic acid and combining fluorescent quantitative PCR (Polymerase Chain Reaction) detection

The invention relates to a method for pre-amplifying multi-target nucleic acid and combining fluorescent quantitative PCR (Polymerase Chain Reaction) detection. Wherein the target nucleic acids can be different types of nucleic acids or a mixture of different types of nucleic acids, the number of the target nucleic acids can be greater than 5, 10, 20, 50, 100 and 200, and the method for performing single or multiple fluorescent quantitative PCR porous detection on all the target nucleic acids after pre-amplification on the pre-amplification product is applied to the fields of pathogen detection, mutation detection, drug screening and the like. The multiple pre-amplification multi-target nucleic acid combined single or multiple fluorescent quantitative PCR detection method can selectively amplify multiple targets, and has the advantages of high sensitivity, strong specificity and small sample dosage. The kit has application value in detection, diagnosis and treatment of diseases. 本发明涉及一种预扩增多靶核酸结合荧光定量PCR检测的方法。其