High-efficiency rolling circle amplification method based on hexamer random primer

The invention discloses an efficient rolling circle amplification method based on a hexamer random primer, and relates to the field of molecular biology, nucleic acid amplification and biological detection. Carrying out sulfo modification on the single-stranded DNA, and then cyclizing; bringing the single-stranded circular DNA into contact with a reaction solution comprising a DNA polymerase, a molecular crowding agent, deoxyribonucleoside triphosphate, and a primer having a 3'end and a 5 'end; the single-stranded circular DNA is amplified through rolling circle amplification to form an amplified DNA product, and the method comprises the steps of designing a hexamer random primer, preparing a rapid detection system and a tubular amplification detection method, the primer is designed as a non-specific primer, the hexamer random primer can be theoretically combined with any site of a circular template, and the single-stranded circular DNA can be rapidly detected through the tubular amplification detection metho