Active blocking agent for eliminating immune interference of rheumatoid factors and preparation method of active blocking agent
The invention discloses an active blocking agent for eliminating immune interference of rheumatoid factors and a preparation method of the active blocking agent, and belongs to the technical field of biotechnology pharmaceuticals. Hybridoma cells are obtained through a hybridoma technology and are s...
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| creator | ZHANG LEI XU QINGYANG LIU WENTING YU CHUNYUN MIAO LIANJUN TSUNEI AKIRA |
| description | The invention discloses an active blocking agent for eliminating immune interference of rheumatoid factors and a preparation method of the active blocking agent, and belongs to the technical field of biotechnology pharmaceuticals. Hybridoma cells are obtained through a hybridoma technology and are subjected to multiplication culture, and after cells are collected, the cells are resuspended through a serum-free culture medium to obtain the active blocking agent. Inoculating into a fermentation tank, entering a mammalian cell fermentation mode, fermenting, purifying to obtain an active antibody, changing the solution into a PBS (Phosphate Buffer Solution), carrying out sterile filtration by using a filter membrane, collecting the filtrate subjected to sterile filtration to obtain the active blocking agent, and storing at the temperature of-20 DEG C; the active blocking agent shows good affinity for both IgA-RF and IgM-RF, can block two types of RF at the same time, shows a wider application range, and still can |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE |
| title | Active blocking agent for eliminating immune interference of rheumatoid factors and preparation method of active blocking agent |
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