Method for determining content of dipalmitoyl hydroxyproline

The invention discloses a content determination method of dipalmitoyl hydroxyproline, and belongs to the technical field of amino acid content. The method for determining the content of dipalmitoyl hydroxyproline comprises the following steps: S1, preparing a reference solution; s2, preparing a test solution; and S3, injecting the reference substance solution and the test solution into liquid chromatography for detection, recording a chromatogram, and calculating to obtain the content of dipalmitoyl hydroxyproline. According to the method for determining the content of dipalmitoyl hydroxyproline, through control of a specific octadecylsilane chemically bonded silica stationary phase and a mobile phase of an acetonitrile-phosphoric acid aqueous solution, the detection peak pattern and the separation degree are greatly improved, the chromatographic peak symmetry factor is 0.96-0.99, the detection accuracy is improved, the method further has good repeatability, and the method is suitable for industrial productio