Phosphamide deoxynucleotide and application thereof

The invention belongs to the technical field of molecular biology, and particularly relates to phosphamide deoxynucleotide and application thereof. The phosphamide deoxynucleotide has the following structural formula, R is the application of the phosphamide deoxynucleotide in biosynthesis for the first time, the phosphamide deoxynucleotide serving as a substrate can be synthesized into long-fragment DNA in various PCR reactions, and compared with existing dNTP, the heat stability of the phosphamide deoxynucleotide is remarkably improved. In addition, by adopting the phosphamide deoxynucleotide as the synthetic substrate, the non-specific amplification is less, and the product is purer. 本发明属于分子生物学技术领域，具体涉及一种磷酰胺类脱氧核苷酸及其应用。一种磷酰胺类脱氧核苷酸，其具有如下结构式：其中，R为本发明首次提出了磷酰胺类脱氧核苷酸在生物合成中的应用，作为底物，在各种PCR反应中，可以合成长片段DNA，与现有的dNTP相比，热稳定性显著提高。并且，采用该磷酰胺类脱氧核苷酸作为合成底物非特异性扩增少，产物更纯净。